We narrowed to 2,128 results for: D10

1. pSN879

   Plasmid

   #190693

   Purpose

   Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

   Depositor

   Shinsuke Niwa

   Article

   Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

   Insert

   mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112215

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mutation

   D10A,H840A,N863A,C710S

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-TNFRSF9-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156556

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TNFRSF9 (TNFRSF9 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   Aug. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

   Plasmid

   #102421

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Modified at the Dharmacon SMARTpool siRNA #2 targ…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLY164

   Plasmid

   #184140

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R3, PrhaB, and Ptet

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY305

   Plasmid

   #184159

   Purpose

   Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   mutsfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   K140N, Mutations D10A and H840A on WT cas9. Synon…

   Promoter

   Pcon, PpspA-zraP-22, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-GCN5 core

   Plasmid

   #179551

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-KIF5A-HA-IRES-Puro

    Plasmid

    #166952

    Purpose

    Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129041

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLY54

    Plasmid

    #130923

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

    Plasmid

    #113162

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyso-InPTapp

    Plasmid

    #236000

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-InPTapp (PLEKHA1, LAMP1 Synthetic, Human)
    

    Use

    Tags

    Citrine, Full-length LAMP1, and truncECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Lyso-INPP5E

    Plasmid

    #236004

    Purpose

    Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Lyso-INPP5E (LAMP1, INPP5E Synthetic, Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

    Plasmid

    #223123

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. STIM1-RspA-NFAST

    Plasmid

    #233606

    Purpose

    Expression of hSTIM1-RspA-NFAST

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EFS-SpdCas9-Dnmt3A/3L-V1

    Plasmid

    #222498

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EFS-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222499

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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