We narrowed to 3,529 results for: golden gate

1. MbCas12a_N1153_GPG_GR2

   Plasmid

   #231578

   Purpose

   Encodes a cortisol inducible variant of MbCas12a

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   MbCas12a with a GR2 insertion behind N1153
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMK83

   Plasmid

   #226793

   Purpose

   Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B

   Depositor

   David Nielsen

   Article

   Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

   Insert

   slr0214 (slr0214 Synechocystis sp. PCC 6803)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tetracycline resistance cassette promoter (consti…

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGA016

   Plasmid

   #228231

   Purpose

   Level 0 for 5xOp (GreenGate A module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   5xOp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCHW111111

   Plasmid

   #222456

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-miniDFR (Solanum lycopersicum)

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHW111112

   Plasmid

   #222457

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-miniDFR::LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-miniDFR

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHW00015

   Plasmid

   #222446

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP64-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCHW91069

   Plasmid

   #222462

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-miniDFR::mCherry2-HyP5SM-LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-miniDFR (Solanum lycopersicum)

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHW101008

   Plasmid

   #222453

   Purpose

   Level 1, Position 5 containing CBS4-miniDFR::OsL5::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-miniDFR::OsL5::NOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCHW00014

   Plasmid

   #222445

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP16-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCHW110034

    Plasmid

    #222443

    Purpose

    Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    LoxP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL2142 MTK3-sfGFP

    Plasmid

    #226661

    Purpose

    MTK3 part containing the coding sequence of sfGFP

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPOM034_P7_3"Lys3

    Plasmid

    #216459

    Purpose

    3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    3''lys3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPOM038_P8b_5'Lys3

    Plasmid

    #216463

    Purpose

    5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    5'lys3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTBL2368 MTK PB puro backbone

    Plasmid

    #226673

    Purpose

    Empty MTK PB cargo backbone marked with puromycin resistance.

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJB67

    Plasmid

    #226316

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with K to A CsoS2 MR1-6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K261A, K320A, K380A, K436A, K495A, K546A

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJB74

    Plasmid

    #226314

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y300A, Y359A, Y420A, Y475A, Y534A

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJB73

    Plasmid

    #226313

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y300A, Y359A, Y420A

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJB72

    Plasmid

    #226312

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with Y to A CsoS2 MR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y300A

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJB45

    Plasmid

    #226304

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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