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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,441 results for: dcas9

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Showing: 2001 - 2020 of 2441 results

  11. HD201: pMVP (L3-L2) IRES-eGFP + polyA

    Plasmid
    #121747
    Purpose
    pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRES2-eGFP + polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121747-plasmid-map-sequence-id-237185

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  13. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

    Plasmid
    #121762
    Purpose
    pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    V5 epitope tag + WPRE
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121762-plasmid-map-sequence-id-242653

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  17. II801: pMVP (L5-L4) ecDHFR DD

    Plasmid
    #121721
    Purpose
    pMVP L5-L4 entry plasmid, contains ecDHFR DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term ecDHFR degron domain (stabilized by TMP) to gene of interest.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    ecDHFR Degron Domain (open)
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121721-plasmid-map-sequence-id-237132

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  20. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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Showing: 2001 - 2020 of 2441 results