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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,744 results for: flag

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Showing: 2001 - 2020 of 15744 results

  1. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  12. OPRM1-Tango

    Plasmid
    #66464
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    FLAG and tTA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66464-plasmid-map-sequence-id-157903

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  16. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Michael Cohen
    Publication
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    186791-plasmid-map-sequence-id-368060

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  19. pcDNA3-FLAG-Luc-N

    Plasmid
    #58790
    Purpose
    Express LucN alone in mammalian cells
    Depositor
    Hiroyoshi Ariga
    Publication
    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    firefly Luciferase 1-398
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    T576C,T591C to destroy MfeI and EcoRI sites
    Promoter
    CMV
    Available since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58790-plasmid-map-sequence-id-424721

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Showing: 2001 - 2020 of 15744 results