We narrowed to 4,154 results for: pCMV

1. pCMV-AAAAA-nCas9(D10A)-UGI

   Plasmid

   #187409

   Purpose

   DddA AAAAA variant

   Depositor

   Jin-Soo Kim

   Article

   Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

   Insert

   DddA AAAAA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-BiP-Myc-KDEL T37G

   Plasmid

   #27165

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   ATP bound form: T37G

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV HA hRB delta CDK + T373

   Plasmid

   #58912

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with T373 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCMV-TM-CIBN-BLITz1-TetR-VP16

   Plasmid

   #89878

   Purpose

   expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   TM-CIBN-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV 3xFLAG-LC3B Q116P G120

   Plasmid

   #129289

   Purpose

   Expresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Changed Glutamine 116 to Proline. Deleted amino a…

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

   Plasmid

   #163552

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCMV-intron myc Rab11 S25N

   Plasmid

   #46786

   Purpose

   expression of S25N Rab11a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB11A S25N (RAB11A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N dominant negative

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

10. PCMV-intron myc Rab2 S20N

    Plasmid

    #46780

    Purpose

    expression of S20N Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S20N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-EGFP-T2A-ACREB-WPRE

    Plasmid

    #40867

    Depositor

    Harold Gainer

    Article

    Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

    Insert

    ACREB
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV HA hRB delta CDK + S612

    Plasmid

    #58914

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S612 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCMV HA hRB delta CDK + S608

    Plasmid

    #58913

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S608 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. 3XFlag pCMV5-TOPO TAZ (∂304)

    Plasmid

    #24810

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Contains ONLY amino acids 1-304.

    Promoter

    Available since

    June 3, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 3XFlag pCMV5-TOPO TAZ (106∂)

    Plasmid

    #24814

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-106

    Promoter

    Available since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCMV/myc/mito-mutant-hogg1

    Plasmid

    #18708

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acid 229, Arginine to Glutamine

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-sport2-mGCN5 FU, FLAG Tagged

    Plasmid

    #23098

    Depositor

    Sharon Dent

    Article

    Martinez-Balbas et al EMBO J. 2000 Feb 15. 19(4):662-71.

    Insert

    mouse Gcn5 mRNA (Kat2a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    missing 50 bp upstream of ATG start site.

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCMV WS Full Myc (Wrn cDNA)

    Plasmid

    #19273

    Depositor

    Philip Leder

    Insert

    mouse Wrn cDNA (Wrn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

    Plasmid

    #134333

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge