We narrowed to 14,071 results for: cas9

1. pX330-U6-W308_guide-CBh-hSpCas9

   Plasmid

   #188546

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-U6-L101_guide-CBh-hSpCas9

   Plasmid

   #188547

   Purpose

   Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

   Depositor

   Darrell Kotton

   Article

   Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   T2A-EGFP

   Promoter

   U6

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

   Plasmid

   #222990

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-SauriCas9-KKH-BlastR

   Plasmid

   #220972

   Purpose

   Expresses human codon-optimized SauriCas9-KKH

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9-KKH
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Mutation

   D10A and H839A point mutations

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mPLD3-sgRNA-Cas9-mcherry

   Plasmid

   #199700

   Purpose

   encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AID-C12*-dCas9-BE4max

   Plasmid

   #216731

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-dCas9-BE4max (AICDA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF823_EFS-Cas9-P2A-Puro

    Plasmid

    #211645

    Purpose

    EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCF226_EFS-Cas9-P2A-Puro

    Plasmid

    #211644

    Purpose

    EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cas9-T2A-H2B mCitirine

    Plasmid

    #210471

    Purpose

    Coexpression of Cas9 and H2B mCitrine

    Depositor

    Amro Hamdoun

    Insert

    Cas9-T2A-H2B mCitirine
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCitrine on C terminal of H2B

    Expression

    Mammalian

    Mutation

    Cas9 contains 3xFLAG tag and nuclear localization…

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eA3Ai-Cas9n-UGI-NLS

    Plasmid

    #207165

    Purpose

    Express A3A with an N57G point mutation and an intron in a BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
    

    Tags

    Cas9-UGI--NLS

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDL25_Cas9-His Δcys E1207C

    Plasmid

    #206286

    Purpose

    Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL23_Cas9-His Δcys E945C

    Plasmid

    #206284

    Purpose

    Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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