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pFLAG_OriM

Plasmid

#110097

Purpose

Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP2

Plasmid

#22403

Depositor

Alexandra Newton

Article

Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

Insert

PHLPP2 (PHLPP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMINTC3H

Plasmid

#110075

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Kan)

Plasmid

#110089

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Apr)

Plasmid

#110094

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Hyg)

Plasmid

#110090

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCOND (Hyg)

Plasmid

#110093

Purpose

Suicide vector based on the TetR/Pip OFF system.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltagalK (Apr)

Plasmid

#110091

Purpose

Analogous to pKO but without the negative selection gene galK.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKOdeltasacB (Kan)

Plasmid

#110092

Purpose

Analogous to pKO but without the negative selection gene sacB.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMcSrc

Plasmid

#17672

Depositor

David Shalloway

Article

Johnson et al Mol Cell Biol. 1985 May . 5(5):1073-83.

Insert

c-src (SRC Chicken)

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pJK599

Plasmid

#72524

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Mutation

synthetic gene

Promoter

T7 and n/a

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJK618

Plasmid

#72535

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)

Depositor

T. J. Kappock

Inserts

formylglycinamidine ribonucleotide synthetase, small subunit
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit

Tags

His6

Expression

Bacterial

Mutation

synthetic gene

Promoter

T7 and n/a

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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YSBE 731 : pHYG_4xFLAG

Plasmid

#92090

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

4xFlag

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKO (Kan)

Plasmid

#110086

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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