We narrowed to 295,319 results for: des
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Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.2-V5-DEST-hSFRP1(aa53-166)
Plasmid#189544PurposeProtein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-166.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
UseTagsExpressionMammalianMutationcontains aa53-166PromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-hSFRP1(aa181-314)
Plasmid#189548PurposeProtein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
UseTagsGSTExpressionMammalianMutationcontains aa181-314PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-hSFRP1(aa53-169)
Plasmid#189545PurposeProtein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
UseTagsGSTExpressionMammalianMutationcontains aa53-169PromoterAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mNeonGreen-stop
Plasmid#186392PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-miRFP713-stop
Plasmid#186391PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mEos4b-stop
Plasmid#186390PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mClover3-stop
Plasmid#186389PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Rfa-mCherry-stop
Plasmid#186388PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mVenus-RfA
Plasmid#186384PurposeDestination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mScarlet-I-RfA
Plasmid#186383PurposeDestination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mNeonGreen-RfA
Plasmid#186382PurposeDestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mEos4b-RfA
Plasmid#186380PurposeDestination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mClover3-RfA
Plasmid#186379PurposeDestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mCherry-RfA
Plasmid#186378PurposeDestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM262 - pDESTR4-R3 mosTI hygroR target
Plasmid#159840PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpDESTR4-R3 mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-M135R-EGFP
Plasmid#164944PurposeTol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deleted; M135RPromoteractinb (actb2) promoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
Plasmid#164945PurposeTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFPā. This will reflect the linkage of KCNJ13 and EGFPDepositorInsertKCNJ13 (KCNJ13 Human)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-Q153H-EGFP
Plasmid#164943PurposeTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154IDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deleted; Q153H; M154IPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-T131A-EGFP
Plasmid#164942PurposeTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deleted; T131A; G133RPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj1b-IRES-EGFP
Plasmid#164946PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFPDepositorInsertkcnj1b (kcnj1b Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-cjSTELLA-mCerulean-PuroTK
Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS
Plasmid#126203PurposeMammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIC3Nme
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1149-pCSDest-AcrIIA4-mCherry-miR122BS
Plasmid#126205PurposeMammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIA4
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-GABARAPL1 G116
Plasmid#123119PurposeExpresses EGFP-GABARAPL1 G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Hemmar-DSCP-ftz-FLP(D)
Plasmid#119894PurposeFLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intronDepositorInsertsUseDrosophila expressionTagsExpressionMutationa mutated form of FLP, which at position 5 contaiā¦PromoterAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES
Plasmid#113439PurposeExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I no force control (F40-based)
Plasmid#118726PurposeThe no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationtruncation after aa1952PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala7-Flag
Plasmid#41010DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsFlagExpressionMammalianMutationchanged amino acids 1189-1192 to AlaninePromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only