Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,493 results for: D10

Showing: 2101 - 2120 of 2493 results

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13220

Plasmid

#219895

Purpose

The base plasmid of TUNEYALI for TF30

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30

Expression

Yeast

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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BPK617

Plasmid

#179297

Purpose

Expresses dCas9 fused to VP64

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_iRFP713

Plasmid

#167868

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Hygro

Plasmid

#167867

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EBFP2

Plasmid

#167865

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTHAP12-N1.1.2-gDNA

Plasmid

#175860

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

THAP12-Nickase2

Use

CRISPR

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCHCHD3-N2.1.2-gDNA

Plasmid

#175858

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CHCHD3-Nickase2

Use

CRISPR

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCHCHD3-N1.1.1-gDNA

Plasmid

#175857

Purpose

CRISPR/Cas9 plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

CHCHD3-Nickase1

Use

CRISPR

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9n-EGFP-L2

Plasmid

#141044

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9n-2A-EGFP

Expression

Mammalian

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-L

Plasmid

#122343

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-R

Plasmid

#122344

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Npm1-R

Plasmid

#122331

Purpose

Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Npm1

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Npm1-L

Plasmid

#122332

Purpose

Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Npm1

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dhx15-R

Plasmid

#122335

Purpose

Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Dhx15

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Dhx15-L

Plasmid

#122336

Purpose

Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx15

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Birc6-L

Plasmid

#122337

Purpose

Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Birc6

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Birc6-R

Plasmid

#122338

Purpose

Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Birc6

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Trim28-R

Plasmid

#122339

Purpose

Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Trim28

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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