We narrowed to 5,724 results for: Chia

1. plYJP022-int7

   Plasmid

   #202287

   Purpose

   Plasmid containing integrase for LPattB7 site

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   int7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2CT-His-MBP-Lbu_C2c2_WT

   Plasmid

   #83482

   Purpose

   Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2CT-His-MBP-Lwa_Cas13a_WT

   Plasmid

   #91865

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LwaCas13a
   

   Use

   CRISPR

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJM.771

   Plasmid

   #108534

   Purpose

   NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   NahRAM + PSalTTC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NahR: I25V P72H V118A R248C

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScalps_Puro

   Plasmid

   #99636

   Purpose

   Empty backbone, 3rd generation lentiviral vector

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   SFFVp

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE30-His-PheRS

   Plasmid

   #124116

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. G-CaMP3

    Plasmid

    #22692

    Purpose

    a single-wavelength GCaMP2-based genetically encoded calcium indicator

    Depositor

    Loren Looger

    Article

    Tian et al Nat Methods. 2009 Dec;6(12):875-81.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N363D mutation (increased fluorescence)

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-ZoAl-ENT-3xF-ORF

    Plasmid

    #238484

    Purpose

    For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement mRNA
    

    Use

    Ivt template

    Tags

    3x-Flag

    Expression

    Mutation

    R1103A

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-QFGal4-SV40pA

    Plasmid

    #155118

    Purpose

    Middle entry vector containing QFGal4

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDIAI

    Plasmid

    #51655

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    apramycin antibiotic resistance gene
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF464

    Plasmid

    #167672

    Purpose

    Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJM253 (miniCTX1-rhaSR-PrhaBAD)

    Plasmid

    #110571

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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