We narrowed to 2,594 results for: gem

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SMT000

Plasmid

#216849

Purpose

Plasmid that encodes a sfGFP gene under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramphenicol resistant.

Depositor

Polly Fordyce

Article

Thompson et al Adv Mater Interfaces 2024, 12 (3), 24004

Insert

Superfolder Green Fluorescent Protein

Use

Synthetic Biology

Expression

Bacterial

Mutation

S30R, Y39N, N105T, Y145F, I171V, and A206V

Promoter

T7

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Article

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

none

Promoter

SP6

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-FLAG-dCas9

Plasmid

#190130

Purpose

Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

FLAG

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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JGI_018363

Plasmid

#158859

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Gemmati_022_1075

Use

Synthetic Biology

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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DT039A

Plasmid

#159400

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23117 (weak) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT038A

Plasmid

#159399

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23106 (medium) promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT040A

Plasmid

#159401

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117/J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

hybrid J23117-J23106 promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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JGI_018343

Plasmid

#158849

Purpose

Rare earth element alcohol dehydrogenases

Depositor

Joint Genome Institute

Article

Huang et al ISME J. 2019 Aug;13(8):2005-2017. doi: 1

Insert

Gemmati_022_16911

Use

Synthetic Biology

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr267

Plasmid

#136754

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr267

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr267 (Olfr267 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr71

Plasmid

#136759

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr71

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr71 (Olfr71 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr2

Plasmid

#136760

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr2

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr2 (Or6a2 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr15

Plasmid

#136761

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr15

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr15 (Olfr15 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr536

Plasmid

#136742

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr536

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr536 (Olfr536 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1508

Plasmid

#136746

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1508

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1508 (Or4e1 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr286

Plasmid

#136748

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr286

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr286 (Olfr286 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr288

Plasmid

#136749

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr288

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr288 (Olfr288 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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