We narrowed to 2,545 results for: gem

1. GEM

   Plasmid

   #55680

   Purpose

   expression of mouse GEM

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   GEM (Gem Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

2. GEM

   Plasmid

   #38820

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GEM (Gem Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. EGFP-GEM

   Plasmid

   #111191

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

   Insert

   GEM (GEM Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T2A

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-GEM

   Plasmid

   #187648

   Purpose

   Expresses the rheological probe PfV-HaloTag-SNAP-tag (GEM-HaloTag-SNAP-tag) under control of human synapsin promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Insert

   PfV
   

   Use

   AAV

   Tags

   HaloTag and SNAP-tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMS-II

   Plasmid

   #204765

   Purpose

   GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEmCherry2

   Plasmid

   #118413

   Purpose

   Red fluorescent reporter for assessing CRISPR gRNA activity

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSGEMKv10.1

   Plasmid

   #85704

   Purpose

   Xenopus laevis oocyte expression of Kv10.1

   Depositor

   Luis Pardo

   Article

   Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Xenopus expression

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFP_Gem_pcDNA4_HisMaxC

   Plasmid

   #41653

   Depositor

   Henry Colecraft

   Article

   Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

   Insert

   Gem, GTP binding proteins (RGK) (GEM Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Available Since

   Oct. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pT-GEM(2)

    Plasmid

    #62894

    Purpose

    Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT-GEM(0)

    Plasmid

    #62891

    Purpose

    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mOtop3_pGEMHE

    Plasmid

    #114676

    Purpose

    This construct was used to express mouse Otop3 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT-GEM(1)

    Plasmid

    #62893

    Purpose

    Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Type

    Empty backbone

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    None and T7

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. mOtop2_pGEMHE

    Plasmid

    #114675

    Purpose

    This construct was used to express mouse Otop2 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mOtop1_pGEMHE

    Plasmid

    #114674

    Purpose

    This construct was used to express mouse Otop1 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHEZFCNGA5

    Plasmid

    #69861

    Purpose

    oocyte expression vector containing ZFCNGA5

    Depositor

    Gary Matthews

    Article

    Tetreault et al Biochem Biophys Res Commun. 2006 Sep 22;

    Insert

    ZFCNGA5 (cnga5 Zebrafish)
    

    Use

    Xenopus oocyte expression

    Mutation

    none

    Promoter

    T7

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGEM_ΔndhD2.km

    Plasmid

    #185532

    Purpose

    a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

    Depositor

    Paula Tamagnini

    Article

    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

    Insert

    nptII gene flanked by ndhD2 upstream and downstream regions
    

    Use

    Synthetic Biology

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM_Δflv3.sm

    Plasmid

    #185533

    Purpose

    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

    Depositor

    Paula Tamagnini

    Article

    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

    Insert

    aadA gene flanked by flv3 upstream and downstream regions
    

    Use

    Synthetic Biology

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE:mTREK-1

    Plasmid

    #224743

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Promoter

    T7

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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