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Plasmid#221476PurposeGateway cloning of NKX2-1 C-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 224-371AA C-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_DelNTerm_160-371AA
Plasmid#221475PurposeGateway cloning of NKX2-1 N-terminal truncation mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal domainPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_HD_160-223AA
Plasmid#221477PurposeGateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelNTerm_160-371AA
Plasmid#221487PurposeNKX2-1 N-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-hPGK_NKX2-1_Short_V5-GFP
Plasmid#221498PurposeNKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5-linker-GFPExpressionMammalianMutationPromoterhPGKAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_DelCTerm_1-223AA
Plasmid#221488PurposeNKX2-1 C-terminal truncation mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 224-371AA C-terminal domainPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-BBS8
Plasmid#218716PurposeExpresses N-terminally EGFP-tagged BBS8 in mammalian cellsDepositorInsertBBS8 (TTC8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MP6-SlugABEN-738
Plasmid#214348PurposeExpresses N-terminus of ABE, and untargeted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCas9-P2A-puro
Plasmid#214353PurposeExpresses SlugCas9 and puromycin resistance genesDepositorInsertSlugCas9 and puromycin resistance genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
XPO6-2Strep
Plasmid#210356PurposeExpresses XPO6 fused with 2Strep in mammalian cellsDepositorInsertXPO6 (XPO6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM2-2Strep
Plasmid#210374PurposeExpresses TRAM2 fused with 2Strep in mammalian cellsDepositorInsertTRAM2 (TRAM2 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM1-2Strep
Plasmid#210352PurposeExpresses TRAM1 fused with 2Strep in mammalian cellsDepositorInsertTRAM1 (TRAM1 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorInsertSGTA (SGTA Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro
Plasmid#210856PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationL324D/R543D/R548DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-L476/L479D
Plasmid#210845PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationL476/L479DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (450-517)-A452D/F453D
Plasmid#210851PurposeMammalian expression of ATG13 (450-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationA452D/F453DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (363-450)
Plasmid#210849PurposeMammalian expression of ATG13 (363-450) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-F509/F512D
Plasmid#210847PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF509/F512DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-L495/L499D
Plasmid#210846PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationL495/L499DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-ATG13 (460-517)-F466D/F470D
Plasmid#210844PurposeMammalian expression of ATG13 (460-517) mutantDepositorInsertATG13 (ATG13 Human)
UseTagsExpressionMammalianMutationF466D/F470DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE011
Plasmid#207912PurposeLevel 0 for gRNA in C4 module (5' GCAG-3' TCAG)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS018
Plasmid#207911PurposeLevel 0 for gRNA in C3 module (5' TTCG-3' GCAG)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS017
Plasmid#207909PurposeLevel 0 for gRNA in C1 module (5' GGCT-3' AGCC)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS020
Plasmid#207915PurposeLevel 0 for gRNA in D3 module (5' TCCC-3' CTGC)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE012
Plasmid#207914PurposeLevel 0 for gRNA in AD2 module (5' TGAC-3' TCCC)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE010
Plasmid#207910PurposeLevel 0 for gRNA in C2 module (5' AGCC-3' TTCG)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS019
Plasmid#207913PurposeLevel 0 for gRNA in D1 module (5' TCAG-3' TGAC)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
H3-pAc5.1 OST4 mCherry Oskar 139-606
Plasmid#206494PurposeOskar 139-606 expression for Schneider cell imagingDepositorInsertOskar 139-606
UseTagsOST4 mcherryExpressionInsectMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHWBF09exp
Plasmid#130833PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2del4
Plasmid#130837PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acid residuesPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1delTM
Plasmid#130841PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKG1delKASH
Plasmid#130842PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKG1
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domainPromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP2del4ec
Plasmid#130843PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseUnspecifiedTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acid residuesPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1del4ec
Plasmid#130844PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acid residuesPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKP1delKASHec
Plasmid#130845PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of KASH domain, addition of three alanin…PromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKG1delKASHec
Plasmid#130846PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKG1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of KASH domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits