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HisMS2_VLP_RSV

Plasmid

#175958

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Inserts

P gene
M gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HisMS2_VLP_RSV

Plasmid

#175958

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Inserts

P gene
M gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FusX alphaDdCBE 1397C

Plasmid

#221936

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE 1397N

Plasmid

#221937

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX alphaDdCBE 1397C

Plasmid

#221936

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX alphaDdCBE 1397N

Plasmid

#221937

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HO-pMET-poly-KanMX4-HO

Plasmid

#51665

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HO-pMET-poly-KanMX4-HO

Plasmid

#51665

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13318 - pAAV-AiE0390m_3xC2-minBG-SYFP2-4x2C-WPRE3-BGHpA (Alias: CN3318)

Plasmid

#208167

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13318 - pAAV-AiE0390m_3xC2-minBG-SYFP2-4x2C-WPRE3-BGHpA (Alias: CN3318)

Plasmid

#208167

Purpose

AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

Depositor

Allen Institute for Brain Science, Jonathan Ting

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-mCA1-emptyMCS

Plasmid

#35095

Depositor

James Fleet

Article

Xue et al Mol Cancer Res. 2010 Aug;8(8):1095-104.

Type

Empty backbone

Use

Mouse Targeting

Promoter

mouse carbonic anhydrase 1

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pUC-mCA1-emptyMCS

Plasmid

#35095

Depositor

James Fleet

Article

Xue et al Mol Cancer Res. 2010 Aug;8(8):1095-104.

Type

Empty backbone

Use

Mouse Targeting

Promoter

mouse carbonic anhydrase 1

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHBFD1

Plasmid

#53565

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HisMS2_VLP_SC2

Plasmid

#175957

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the SARS-CoV-2 N gene [CoV USA/WA-CDC-WA1/2020]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

N gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HisMS2_VLP_EN_SC2

Plasmid

#175959

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the SARS-CoV-2 E and N genes [CoV USA/WA-CDC-WA1/2020]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

E gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HisMS2_VLP_SC2

Plasmid

#175957

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the SARS-CoV-2 N gene [CoV USA/WA-CDC-WA1/2020]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

N gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HisMS2_VLP_EN_SC2

Plasmid

#175959

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the SARS-CoV-2 E and N genes [CoV USA/WA-CDC-WA1/2020]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

E gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge