We narrowed to 5,842 results for: pCas

1. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-TWIN-STREP-FLAG-NPRL2

   Plasmid

   #164985

   Purpose

   NPRL2 expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   NPRL2 (NPRL2 Human)
   

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS SARS-CoV-2 BA.2.75 Spike

   Plasmid

   #190012

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike

   Depositor

   Marceline Côté

   Article

   Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

   Insert

   SARS-CoV-2 BA.2.75 Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the BA.2 mutations (T19I, LPPA24S, G142D…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   Sept. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCAGGS-Flag-hsDicer (D1320A/D1709A)

   Plasmid

   #41585

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D1320A/D1709A

   Promoter

   CMV

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCAG-2dV-Camui (T305D/T306D)

   Plasmid

   #220368

   Purpose

   T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed both Threonine 305 and 306 to aspartic ac…

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

   Plasmid

   #87109

   Purpose

   BPNLS-Cas9-BPNLS-2AGFP expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS-2A-EGFP
   

   Use

   CRISPR

   Tags

   3xFLAG (on Cas9)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-lox-roxSTOProx-tRFP-pA-lox

   Plasmid

   #196888

   Purpose

   Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   turbo (t)RFP
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEN716 - pCAGGS-eGFP-3Xnls-bpA

   Plasmid

   #156435

   Purpose

   For transient expression of nuclear eGFP in mammalian cells

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   eGFP
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

    Plasmid

    #138295

    Purpose

    gRNA for CRISPR/Cas9 knockout of human TRIM21

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    gRNA TRIM21 (TRIM21 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

    Plasmid

    #185676

    Purpose

    SpCas9 and gRNA targeting the C-terminus of HsIRE1

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 gRNA (ERN1 Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-TWIN-STREP-FLAG-NPRL3

    Plasmid

    #164984

    Purpose

    NPRL3 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    NPRL3 (NPRL3 Human)
    

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

    Plasmid

    #80769

    Purpose

    Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    IFT88 gRNA#1 (IFT88 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCalpha EV (PKA catalytic subunit Calpha)

    Plasmid

    #15310

    Depositor

    Stanley McKnight

    Article

    Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

    Insert

    PRKACA (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pU6-PspCas13b-non-targeting (pJK5)

    Plasmid

    #229052

    Purpose

    Cloning backbone for PspCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-Golden-Gate-Esp3I-Destination

    Plasmid

    #51278

    Purpose

    CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS SARS-CoV-2 BA.4.6 Spike

    Plasmid

    #190013

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4.6 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4.6 Spike (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    Sept. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pOTTC1124 - pAAV MeCP2 SpCas9(D10A)

    Plasmid

    #112719

    Purpose

    An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    AAV

    Mutation

    D10A

    Promoter

    Mecp2

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge