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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 2141 - 2160 of 2672 results

  3. pEPQD1CB0202

    Plasmid
    #185626
    Purpose
    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter
    Depositor
    Nicola Patron
    Publication
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR and Synthetic Biology
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185626-plasmid-map-sequence-id-368553

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  9. pSL1166

    Plasmid
    #129520
    Purpose
    pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation
    Depositor
    Scott Lindner
    Publication
    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    Plasmodium, rodent-infectious species
    Tags
    3XFLAG-NLS
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129520-plasmid-map-sequence-id-256186

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  13. pCBS-4559

    Plasmid
    #249331
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAs
    Depositor
    Chase Beisel
    Publication
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249331-plasmid-map-sequence-id-497004

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  14. pCBS-5391

    Plasmid
    #249330
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cells
    Depositor
    Chase Beisel
    Publication
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249330-plasmid-map-sequence-id-497003

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  15. pYPQ265E7

    Plasmid
    #213470
    Purpose
    Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing
    Depositor
    Yiping Qi
    Publication
    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:
    Insert
    TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
    Use
    CRISPR; Gateway compatible tada-cdd-32aa linker-z…
    Tags
    No
    Expression
    Plant
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213470-plasmid-map-sequence-id-426727

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  18. pCBS-5846

    Plasmid
    #249329
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cells
    Depositor
    Chase Beisel
    Publication
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249329-plasmid-map-sequence-id-497002

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  19. pCBS-6756

    Plasmid
    #249328
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells
    Depositor
    Chase Beisel
    Publication
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249328-plasmid-map-sequence-id-497001

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  20. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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Showing: 2141 - 2160 of 2672 results