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pAc5.1B-lambdaN-HA-DmNot1-3xL-Amut-dsRNAres_P

Plasmid

#147165

Purpose

Insect Expression of DmNot1-3xL-Amut-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmNot1-3xL-Amut-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmAgo1_572-984-optim_K

Plasmid

#146728

Purpose

Bacterial Expression of DmAgo1_572-984-optim

Depositor

Elisa Izaurralde

Insert

DmAgo1_572-984-optim (AGO1 Fly)

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-V5His6_D

Plasmid

#146095

Purpose

Insect Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Insect

Mutation

seven silent mutations compared to the sequence g…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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AByH

Plasmid

#164152

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByD

Plasmid

#164150

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByC

Plasmid

#164149

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByB

Plasmid

#164148

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByA

Plasmid

#163749

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRVV653

Plasmid

#89474

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRVV598

Plasmid

#87629

Purpose

Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked alleles.

Depositor

Richard Mann

Article

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attP

Use

CRISPR

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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AByI

Plasmid

#164153

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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SCP1-pUC119

Plasmid

#21846

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-pUC119

Use

Puc derivative - a cloning vector

Available Since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pBPZpGAL4DBDUw

Plasmid

#26233

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

Zip-GAL4DBD

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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No-repeats

Plasmid

#122487

Purpose

A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Insert

LacZ

Expression

Insect

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMycDam

Plasmid

#59218

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Promoter

Drosophila heat-shock promoter

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJFRC-MUH

Plasmid

#26213

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Type

Empty backbone

Expression

Insect

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

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