We narrowed to 4,090 results for: biorxiv

1. pPB-cT3G-cERP2-3xFLAG-SMAD9-active

   Plasmid

   #222580

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   SMAD9 (SMAD9 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   S489E, S491E

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-U6.3-sgRNA-non-targeting-control -GFP

   Plasmid

   #221844

   Purpose

   Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Inserts

   EGFP
   non-targeting control sgRNA- GCACTGCTACGATCTACACC
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Expression

   Mammalian

   Promoter

   GACG and U6.3 chick

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-GST-ATG13 (363-517)

   Plasmid

   #222823

   Purpose

   Bacteria expression of His-GST -ATG13 C-terminal

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   6His-GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18-LucFF

   Plasmid

   #220496

   Purpose

   To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   Firefly Luciferase
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLX-SFFV_NKX2-1_Short_V5-GFP

   Plasmid

   #221500

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-EF1a_NKX2-1_CdTag-HA

   Plasmid

   #221493

   Purpose

   NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-EF1a_NKX2-1_W208L

   Plasmid

   #221486

   Purpose

   NKX2-1 W208L DNA binding mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   W208L

   Promoter

   EF1a

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-UBC_NKX2-1

   Plasmid

   #221495

   Purpose

   NKX2-1 short isoform lentiviral overexpression using UBC promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_NKX2-1_DelCTerm_1-223AA

   Plasmid

   #221476

   Purpose

   Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Mutation

   Deletion of 224-371AA C-terminal domain

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221475

    Purpose

    Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Mutation

    Deletion of 1-159AA N-terminal domain

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221487

    Purpose

    NKX2-1 N-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX-EF1a_NKX2-1_V205F

    Plasmid

    #221485

    Purpose

    NKX2-1 V205F DNA binding mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    V205F

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX-hPGK_NKX2-1_Short_V5-GFP

    Plasmid

    #221498

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX-EF1a_NKX2-1_DelCTerm_1-223AA

    Plasmid

    #221488

    Purpose

    NKX2-1 C-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 224-371AA C-terminal domain

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX-SFFV_NKX2-1

    Plasmid

    #221496

    Purpose

    NKX2-1 short isoform lentiviral overexpression using SFFV promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDRF1-GW mKelly-T2A-mtq2

    Plasmid

    #205506

    Purpose

    Expression of mKelly-T2A-mtq2 in yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mKelly-T2A-mtq2
    

    Expression

    Yeast

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. dSp-Rta Full (1-190)

    Plasmid

    #99662

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    truncated RTA activation domain (1-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218637

    Purpose

    Proximity biotinylation control of OSBP(PH domain)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    PH domain only

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-MBP-TEVpCS-ADGRF2 CTF-FLAG

    Plasmid

    #217695

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF2 (ADGRF2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-429

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Efg1-ΔDBD-A51P/A56P

    Plasmid

    #216400

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P
    

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51 and 56 to Proline, and deleted…

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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