Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 8,997 results for: ef1;

Showing: 2161 - 2180 of 8997 results

  4. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

    Enlarge

  6. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

    Enlarge

  10. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Expression
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

    Enlarge

  11. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51094
    Purpose
    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    oChIEF(E163A/T199C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E163A/T199C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51094-plasmid-map-sequence-id-79315

    Enlarge

  12. AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA

    Plasmid
    #51095
    Purpose
    Cre dependent expression of ChIEF kinetic variant E162A/T198C with physically uncoupled dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    ChIEF(E162A/T198C)
    Use
    AAV
    Tags
    P2A-dTomato
    Expression
    Mammalian
    Mutation
    E162A/T198C
    Promoter
    EF1a
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51095-plasmid-map-sequence-id-79317

    Enlarge

  13. pJM671 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 in TUPV3

    Plasmid
    #161573
    Purpose
    Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6 under the EF1α promoter
    Depositor
    Joshua Leonard
    Article
    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe
    Insert
    TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF6
    Use
    Synthetic Biology
    Tags
    3x-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    161573-plasmid-map-sequence-id-317930

    Enlarge

  14. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid
    #176461
    Purpose
    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176461-plasmid-map-sequence-id-346306

    Enlarge

  16. pJM612 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 in TUPV3

    Plasmid
    #161571
    Purpose
    Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1 under the EF1α promoter
    Depositor
    Joshua Leonard
    Article
    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe
    Insert
    TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-VP64-ZF1
    Use
    Synthetic Biology
    Tags
    3x-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    161571-plasmid-map-sequence-id-317637

    Enlarge

  17. pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

    Plasmid
    #229744
    Purpose
    Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.
    Depositor
    Shawn Ferguson
    Article
    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Tags
    Halo
    Expression
    Mammalian
    Mutation
    LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA
    Promoter
    EF1A
    Available since
    Feb. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    229744-plasmid-map-sequence-id-462110

    Enlarge

  18. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid
    #201539
    Purpose
    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Inserts
    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201539-plasmid-map-sequence-id-401324

    Enlarge

  19. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid
    #131684
    Purpose
    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1
    Depositor
    Christopher Richie
    Article
    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10
    Inserts
    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    EF1a and mU6-LSL (Cre dependent)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    131684-plasmid-map-sequence-id-437146

    Enlarge

  20. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

    Enlarge

Showing: 2161 - 2180 of 8997 results