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pET28a-PvuRts1I

Plasmid

#86888

Purpose

express C-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

C-term His tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-E71A-PvuRts1I

Plasmid

#86895

Purpose

express E71A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

E71A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-D57A-PvuRts1I

Plasmid

#86892

Purpose

express D57A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

D57A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-R208A-PvuRts1I

Plasmid

#86891

Purpose

express R208A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

R208A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-E20A-PvuRts1I

Plasmid

#86897

Purpose

express E20A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

Pvurts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

E20A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-D70A-PvuRts1I

Plasmid

#86890

Purpose

express D70A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

His

Expression

Bacterial

Mutation

D70A

Promoter

T7

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-PvuRts1I

Plasmid

#86887

Purpose

express N-term His tagged PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDHJS4 AN-

Plasmid

#78247

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AN+

Plasmid

#78246

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pQuantA-hygro-kana

Plasmid

#45585

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

Oct. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pJK398

Plasmid

#155370

Purpose

dCas9 sgRNA oscillator v1 (dCRv1) (p15A)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

dCas9 sgRNA oscillator v1

Use

CRISPR

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pJK517

Plasmid

#155385

Purpose

PphlF-TA4 in crRNAs

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Insert

PphlF-TA4 in dCas12a oscillator crRNAs

Use

CRISPR

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP

Plasmid

#129020

Purpose

Expresses eGFP

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBSTNAV

Plasmid

#45801

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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p18S.1 wt

Plasmid

#51727

Purpose

transcribes wild type 18S without modifications

Depositor

Vincent Mauro

Article

Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

Insert

18S

Promoter

pol-I

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) mCherry

Plasmid

#176301

Purpose

Expresses mCherry mRNA from CMV promoter

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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p44K

Plasmid

#45800

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge