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pET-His-SUMO-LS4

Plasmid

#228450

Purpose

LS4 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS4

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-SUMO-LS12

Plasmid

#228451

Purpose

LS12 protein expression vector for bacteria

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

LS12

Tags

10xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS1M2b-3c-ON8-EGFP

Plasmid

#228452

Purpose

LS4-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS1M2b-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-CS2-3c-ON8-EGFP

Plasmid

#228453

Purpose

LS12-responsive riboswitch

Depositor

Keisuke Fukunaga

Article

Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

CS2-3c-ON8 riboswitch-regulated EGFP

Use

Synthetic Biology

Promoter

T7

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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Const.2 EGFP-P2A-dTomato-PS9

Plasmid

#231906

Purpose

For packaging EGFP and dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5-1x-uORF

Plasmid

#229172

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

1x uORF

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5-2x-uORF

Plasmid

#229173

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

2x uORF

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5-CaseR

Plasmid

#229169

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

CasE restiction site

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-T-Rb-Glob-pA

Plasmid

#229193

Purpose

lvl 0 Terminator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Rb-Glob-pA

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-5-1x-wuORF

Plasmid

#229171

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

1x weak uORF

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-Gal4-VP16

Plasmid

#229185

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Gal4-VP16

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-Gal4-VP64

Plasmid

#229186

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Gal4-VP64

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) K250A

Plasmid

#192052

Purpose

Expresses a DNA binding mutant K250A (ORF37)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (K250A)

Use

Unspecified

Mutation

DNA binding mutant K250A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) D221N E244Q

Plasmid

#192053

Purpose

Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (D221N E244Q)

Use

Unspecified

Mutation

2X catalytic mutant D221N E244Q

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) 318-320A

Plasmid

#192054

Purpose

Expresses a mutant of SOX 318-320A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (318-320A)

Use

Unspecified

Mutation

SOX 318-320A

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAP007

Plasmid

#206809

Purpose

pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expression

Depositor

Daan Swarts

Article

Finocchio et al Nucleic Acids Res. 2024 Feb 28;52(4):201

Insert

BabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)

Tags

6xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTarget-Dual

Plasmid

#207884

Purpose

Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

I-OnuI target site
I-GpeMI target site

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Tags

6x His

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR119

Plasmid

#185051

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L34

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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