We narrowed to 7,746 results for: sms

1. pLC242-GFP-Sestrin2

   Plasmid

   #100519

   Purpose

   Expresses GFP tagged Sestrin2 in mammalian cells to make lentivirus

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SESN2 (SESN2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCGN-ELK-1 Ets mut

   Plasmid

   #27157

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R62E/R65E/Y66D

   Promoter

   Available since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pTW194b63

   Plasmid

   #136930

   Purpose

   Directed evolution of SpCas10

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   npuC-gIII(C)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTU2S-a (colE1 origin)

   Plasmid

   #74091

   Purpose

   Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. eTC GFP ß-actin ΔZip

   Plasmid

   #27124

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Use

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   This construct lacks β-actin 3′UTR and therefore …

   Promoter

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXL001

   Plasmid

   #26122

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   tTR-KRAB
   

   Use

   Lentiviral

   Tags

   IRES-Puro

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2012

   Availability

   Academic Institutions and Nonprofits only

7. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF730

   Plasmid

   #121668

   Purpose

   EF1a-ProCas9-Flavi-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9(C)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a core promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PECAM-H2B-GFP-Hygro

   Plasmid

   #14684

   Depositor

   Victoria Bautch

   Article

   Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

   Insert

   PECAM promoter/enhancer (Pecam1 Mouse)
   

   Use

   Tags

   H2B-GFP and PGK-Hygro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. JP1520 PIK3CA H1047R HA

    Plasmid

    #14572

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA H1047R (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

11. px459-RhebL1 sgRNA

    Plasmid

    #133769

    Purpose

    Expresses Cas9 and human RhebL1 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNC1136

    Plasmid

    #41562

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiGuide_GFP_sgEPAS1#1

    Plasmid

    #183119

    Purpose

    pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEK20

    Plasmid

    #210582

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    BdGAPDHpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCF708

    Plasmid

    #121685

    Purpose

    EF1a-dTEV-protease-mTagBFP2

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    TEV protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p156 pWZL-EGFP-SLPI

    Plasmid

    #11069

    Depositor

    Ramesh Shivdasani

    Article

    Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

    Insert

    SLPI (Slpi Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nup54 no FG-mEGFP494(0)

    Plasmid

    #164269

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP-Rpp20-C1

    Plasmid

    #134541

    Purpose

    Expresses YFP-Rpp20 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp20
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA Raptor mutant 9

    Plasmid

    #8476

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only