We narrowed to 7,789 results for: sms

1. pTW195b63

   Plasmid

   #136931

   Purpose

   Directed evolution of SpCas11

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII(N)-npuN
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFP-mGCN5-C1

   Plasmid

   #134573

   Purpose

   Expresses YFP-GCN5 fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   GCN5 (Kat2a Mouse)
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CP1012-ABEmax

   Plasmid

   #119806

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-FLCN

   Plasmid

   #49173

   Purpose

   Mammalian expression of mouse folliculin (FLCN) fused to GFP

   Depositor

   Shawn Ferguson

   Article

   Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

   Insert

   Folliculin (Flcn Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon deleted

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-GFP-CtIP-T847E

   Plasmid

   #71111

   Purpose

   mammalian expession of GFP-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pNL4-3 envFS eGFP NoStarts

   Plasmid

   #101342

   Purpose

   All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Mutation

   No methonines in Gag

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PECAM-H2B-GFP

   Plasmid

   #14689

   Depositor

   Victoria Bautch

   Article

   Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

   Insert

   PECAM promoter/enhancer (Pecam1 Mouse)
   

   Tags

   H2B-GFP

   Expression

   Mammalian

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET21b-LaG9(S151C)

   Plasmid

   #172490

   Purpose

   Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   LaG9(S151C)
   

   Tags

   6xHis, PPX cleavage site, and pelB leader sequenc…

   Expression

   Bacterial

   Mutation

   S151C

   Promoter

   T7

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCGN-ELK-1 S383A

    Plasmid

    #27160

    Purpose

    For mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domain

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    TAD mutant: S383A

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC1125

    Plasmid

    #41561

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9
    

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CP1249-CBEmax

    Plasmid

    #119804

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1249) -UGI-UGI
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

    Plasmid

    #32479

    Purpose

    Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAML141F-GlyR-IRES-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Promoter

    Synapsin

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. ptdTomato-N1-FLCN

    Plasmid

    #49174

    Purpose

    Mammalian expression of mouse folliculin (FLCN) fused to tdTomato

    Depositor

    Shawn Ferguson

    Article

    Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

    Insert

    Folliculin (Flcn Mouse)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Stop codon deleted

    Promoter

    CMV

    Available Since

    Nov. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T1-Cdc42 G15A

    Plasmid

    #69356

    Purpose

    expression of nucleotide free Cdc42 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pBS NL4-3 envFS eGFP RT-D185K/D186L

    Plasmid

    #101332

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Mutation

    reverse transcriptase D185K/D186L

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-PTEN 3'UTR

    Plasmid

    #28104

    Depositor

    Jeffrey Rosen

    Article

    Greene et al J Cell Sci. 2010 Feb 15. 123(Pt 4):606-1

    Insert

    PTEN 3'UTR (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pYPQ262m3

    Plasmid

    #149556

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. JP1520 PIK3CA E545K HA

    Plasmid

    #14571

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA E545K (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only