We narrowed to 3,795 results for: golden gate

1. pEPOR0SP0009

   Plasmid

   #117522

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-p (no stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL90005

   Plasmid

   #117520

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-h (no stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR0SP0008

   Plasmid

   #117535

   Purpose

   Level0 Golden Gate part: CDS, AsCas12a

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   AsCas12a (with stop codon)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGF004

   Plasmid

   #48845

   Purpose

   Provides a D-alanine resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   p35S::D-AlanineR::t35S
   

   Use

   Golden gate compatible cloning vector

   Mutation

   internal BsaI recognition site in 35S promoter re…

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP1950

   Plasmid

   #222729

   Purpose

   J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PAC_E83_LOV_eGFP

   Plasmid

   #231574

   Purpose

   This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ132B

   Plasmid

   #69282

   Purpose

   Golden Gate entry vector; 2nd gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ131A

   Plasmid

   #69273

   Purpose

   Golden Gate entry vector; 1st gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUS-mCh-sspB

   Plasmid

   #227377

   Purpose

   Condensate module, light-inducible oligomerization IDR

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   FUS IDR
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NLS-GFP-iLID-Ferritin

    Plasmid

    #227376

    Purpose

    Condensate module, light-inducible oligomerization 'core'

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    GFP 'core'
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-UPEmS-tevo

    Plasmid

    #228894

    Purpose

    Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM790

    Plasmid

    #242607

    Purpose

    T-DNA plasmid, Level 2 destination vector, replaces pMIN-VS1

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tad-CBE(ACC2)

    Plasmid

    #235061

    Purpose

    Expresses Tad-CBE(ACC2) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC2)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tad-CBE(ACC3)

    Plasmid

    #235062

    Purpose

    Expresses Tad-CBE(ACC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKG1334_pHarbor-PiggyBac_backbone

    Plasmid

    #239705

    Purpose

    PaqCI Golden Gate assembly plasmid encoding the backbone for PiggyBac-mediated integration

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEED

    Plasmid

    #219658

    Purpose

    Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Empty SEED Cassette (3xP3-dsRed)
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD304 VPR-ZF43

    Plasmid

    #138840

    Purpose

    Constitutive expression of VPR-ZF43 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tad-CBE(TCA3)

    Plasmid

    #235064

    Purpose

    Expresses Tad-CBE(TCA3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(TCA3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH-roGFP2-ORP1

    Plasmid

    #191677

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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