We narrowed to 5,523 results for: golden gate
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Plasmid#138096PurposeGolden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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pYPQ132-STU-Lb
Plasmid#138099PurposeGolden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
PUF Assembly Kit
Plasmid kit#1000000051PurposeAssembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typeBacterial Expression, Mammalian ExpressionEditing typePUFCloning typeGolden GateAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Lb
Plasmid#138105PurposeGolden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Lb
Plasmid#138102PurposeGolden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG458 eSp(1.1) V3
Plasmid#226964PurposeCBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS4
Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSL.R.Ly
Plasmid#227697PurposeReengineered geminiviral replicon vectorDepositorTypeEmpty backboneExpressionPlantAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CIDAR MoClo Extension, Volume I
Plasmid kit#1000000161PurposePlasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).DepositorApplicationCloning and Synthetic BiologyVector typeBacterial ExpressionCloning typeGolden Gate (MoClo)Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi
Plasmid#138106PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-pT
Plasmid#138108PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]
Plasmid#247262PurposeExpress ISCro4 recombinase and bridge RNADepositorInsertpNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
ExpressionMammalianMutationC60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…Available sinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fungal Toolkit for Modular Cloning (FTK)
Plasmid kit#1000000191PurposeSynthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typeFungal ExpressionEditing typeCRISPRCloning typeGolden GateAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP3614-MM168 [pNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)]
Plasmid#247266PurposeExpress ISCro4 recombinaseDepositorInsertpNP3613 - ISCro4 Recombinase (S30T/P54Q/S243H)
ExpressionMammalianMutationS30T/P54Q/S243HAvailable sinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Secretion and Display (YSD) Toolkit
Plasmid kit#1000000240PurposeCollection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.DepositorApplicationCloning and Synthetic BiologyVector typeYeast ExpressionCloning typeGolden Gate (MoClo)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458 eSp(1.1) V3
Plasmid#226963PurposeCBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-TLS-CsALSgR1.1
Plasmid#245877PurposeGolden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequenceDepositorInsertCsALSgR1.1
UseCRISPRExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B-TLS-CsALSgR1.2
Plasmid#245878PurposeGolden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequenceDepositorInsertCsALSgR1.2
UseCRISPR; Golden gate entry vectorExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B-TLS-PtALSgRNA
Plasmid#245881PurposeGolden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene along with TLS mobile RNA sequenceDepositorInsertPtALSgRNA
UseCRISPRExpressionPlantPromoterAtU3Available sinceFeb. 2, 2026AvailabilityAcademic Institutions and Nonprofits only