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pET21-pelB-LaG94-1

Plasmid

#228961

Purpose

For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-PIP74

Plasmid

#196813

Purpose

Contains NET4A promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

NET4A (NET4A Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP52

Plasmid

#196790

Purpose

Contains ARR5 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

ARABIDOPSIS RESPONSE REGULATOR 5

Use

Greengate (gg) entry vector

Mutation

1591 C->G

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP37

Plasmid

#196775

Purpose

Contains LECRK-VI.2 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

L-TYPE LECTIN RECEPTOR KINASE-VI.2

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP11

Plasmid

#196749

Purpose

Contains FER promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

FERONIA (FER Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pACB113

Plasmid

#222212

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

Depositor

Gregg Beckham

Publication

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA(W15C)

Tags

6x His

Expression

Bacterial

Mutation

Changed Tryptophan 15 to Cysteine

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ282 (TsCas12a)

Plasmid

#138114

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized TsCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

TsCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ283 (MlCas12a)

Plasmid

#138115

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MlCas12a without promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

MlCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge