167,948 results

1. hCathepsin B

   Plasmid

   #11249

   Purpose

   Mammalian expression of human cathepsin B

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin B (CTSB Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. RN3P-KDM5B-FLAG

   Plasmid

   #86398

   Purpose

   FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

   Depositor

   Wei Xie

   Article

   Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

   Insert

   KDM5B (Kdm5b Mouse)
   

   Tags

   3x-FLAG

   Expression

   Mammalian

   Promoter

   Lac

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. JAK2 (V617F)-pcw107-V5

   Plasmid

   #64610

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   V617F

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry

   Plasmid

   #176016

   Purpose

   Mammalian expression of mCherry. Parton lab clone DFQ

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   mCherry
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSFFV_sfCherry2(11)_TagBFP

   Plasmid

   #82606

   Purpose

   Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_TagBFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   SFFV

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-Kv2.1 K+ channel [K89/34R] (Recombinant Antibody)

   Antibody

   #180083-rAb

   Purpose

   Anti-Kv2.1 K+ channel (Rat) recombinant mouse monoclonal antibody

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Recommended Applications

   Immunohistochemistry and Western Blot

   Reactivity

   Human, Mouse, and Rat

   Source Species

   Mouse

   Isotype

   IgG2a

   Trial Size

   Available to purchase

   Available Since

   March 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

8. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

   Plasmid

   #224435

   Purpose

   AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mScarlet-KASH
   U6-gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPR-StAR4GN (Lenti)

   Plasmid

   #222693

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

    Plasmid

    #61593

    Purpose

    A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    HA, NLS, and OLLAS tag

    Expression

    Mammalian

    Mutation

    K175R and K736R (deUb mutations)

    Available Since

    Feb. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Membrane protein gRNA library

    Pooled Library

    #113345

    Purpose

    Contains 58,071 gRNAs targeting 6,213 membrane proteins and 500 non-targeting gRNAs

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Species

    Homo sapiens

    Use

    Lentiviral

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

12. pBabe_Hyg_cOVA_T2A_mStrawberry

    Plasmid

    #161737

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM461

    Plasmid

    #108320

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    SacRB
    

    Expression

    Bacterial

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CyanoGate Kit

    Plasmid Kit

    #1000000146

    Purpose

    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.

    Depositor

    Alistair McCormick

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pUCmini-iCAP-PHP.eC

    Plasmid

    #201903

    Purpose

    non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

    Insert

    Synthetic construct isolate AAV-PHP.eC VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-mCherry

    Plasmid

    #50462

    Purpose

    Double floxed mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Promoter

    EF1a

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries

    Pooled Library

    #1000000228

    Depositor

    Raj Chari

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only