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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,038 results for: 272

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Showing: 2241 - 2260 of 6038 results

  8. LSB-hsa-miR-16-5p

    Plasmid
    #103272
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-16-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-16-5p target
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103272-plasmid-map-sequence-id-195593

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  10. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid
    #52725
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12 and 15 to alanines
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52725-plasmid-map-sequence-id-82094

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  13. DRD5-Tango

    Plasmid
    #66272
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD5 (DRD5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66272-plasmid-map-sequence-id-305774

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  20. pMXs-hs-3xHA-7CtoA-LIN-41

    Plasmid
    #52726
    Purpose
    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations of all 7 cysteines in the RING domain and 3 N-terminal HA tags
    Depositor
    Shinya Yamanaka
    Article
    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.
    Insert
    LIN-41 (TRIM71 Human)
    Use
    Retroviral
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    changed cysteines 12, 15, 61, 66, 69, 91, and 94 …
    Available Since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    52726-plasmid-map-sequence-id-82095

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Showing: 2241 - 2260 of 6038 results