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We narrowed to 5,282 results for: golden gate

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Showing: 2241 - 2260 of 5282 results

  5. Yeast Secrete and Detect

    Plasmid kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  7. p-OiRS3GG

    Plasmid
    #98589
    Purpose
    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).
    Depositor
    Lydia Contreras
    Publication
    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552
    Inserts
    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    Expression
    Bacterial
    Promoter
    pBAD and pLtetO
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98589-plasmid-map-sequence-id-194130

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  9. Joint Modular Cloning (JMC) Toolkit

    Plasmid kit
    #1000000233
    Purpose
    Modular cloning chassis and set of functional parts for assembling plant transformation vectors, compatible with MoClo.
    Depositor
    Daniel Voytas
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  15. Yeast GPCR-sensor Toolkit

    Plasmid kit
    #1000000157
    Purpose
    This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 2241 - 2260 of 5282 results