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pYC66

Plasmid

#63929

Purpose

Recipient replicative vector to construct MYC tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

MYC

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pML3

Plasmid

#108341

Purpose

MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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Ube2i

Plasmid

#31420

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2I Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pYC16

Plasmid

#63913

Purpose

Recipient overexpressing vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCS-APTA

Plasmid

#247077

Purpose

pCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

aroL
ppsA
tktA
aroG(fbr)

Expression

Bacterial

Mutation

feedback-inhibition-resistant

Promoter

PLlacO1

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-mGold

Plasmid

#224084

Purpose

Expresses Cyterm-mGold to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Cyterm-mGold

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-Hoxa5

Plasmid

#225109

Purpose

Hoxa5 expression in Chick

Depositor

Polyxeni Philippidou

Article

Kc et al Commun Biol. 2024 Sep 17;7(1):1166. doi:

Insert

Hoxa5 (Hoxa5 Mouse)

Use

Avian expression

Mutation

WT

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-V5-Scip

Plasmid

#225110

Purpose

Scip expression in Chick

Depositor

Polyxeni Philippidou

Article

Kc et al Commun Biol. 2024 Sep 17;7(1):1166. doi:

Insert

Scip (Pou3f1 Mouse)

Use

Avian expression

Mutation

WT

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-FRB

Plasmid

#194455

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FRB (MTOR Human)

Tags

GAG

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAM201

Plasmid

#227660

Purpose

pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

Depositor

Andreas Martin

Article

Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

Insert

rpn5 (RPN5 Budding Yeast)

Tags

3x-FLAG-HRV3C-

Expression

Yeast

Mutation

(amino acids 121-131) VTENKIF mutated to AAAAAAA

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 mVermillion-T2A-mTurquoise2

Plasmid

#219847

Purpose

equimolar expression of mVermillion and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mVermillion-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mGarnet2-T2A-mTurquoise2

Plasmid

#219850

Purpose

equimolar expression of mGarnet2 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mGarnet2-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 StayGold-E138D-T2A-mCherry

Plasmid

#219833

Purpose

equimolar expression of Staygold-E138D and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

StayGold-E138D-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKelly-T2A-mtq2

Plasmid

#205506

Purpose

Expression of mKelly-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mKelly-T2A-mtq2

Expression

Yeast

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0012

Plasmid

#205581

Purpose

HYG(1-52)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0011

Plasmid

#205580

Purpose

NpuDnaE(C)_KAN(193-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0010

Plasmid

#205579

Purpose

KAN(1-192)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0009

Plasmid

#205578

Purpose

NpuDnaE(C)_KAN(132-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0008

Plasmid

#205577

Purpose

KAN(1-131)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-ymScarletI

Plasmid

#205512

Purpose

Expression of ymTq2-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW miFP663-T2A-mtq2

Plasmid

#205504

Purpose

Expression of miFP663-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miFP663-T2A-mtq2

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW emiRFP2-T2A-mtq2

Plasmid

#205497

Purpose

Expression of emiRFP2-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

emiRFP2-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-P2A-ymScarletI

Plasmid

#205500

Purpose

Expression of ymTq2-P2A-ymScarletI in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-P2A-ymScarletI

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW ymTq2-E2A-ymScarletl

Plasmid

#205510

Purpose

Expression of ymTq2-E2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-E2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-O2A-ymScarletl

Plasmid

#205509

Purpose

Expression of ymTq2-O2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-O2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW Achilles-T2A-mCherry

Plasmid

#205499

Purpose

Expression of Achilles-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Achilles-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymCardinal-T2A-mtq2

Plasmid

#205508

Purpose

Expression of ymCardinal-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymCardinal-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFP680-T2A-mtq2

Plasmid

#205502

Purpose

Expression of miRFP680-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP680-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW hfYFP-T2A-mtq2

Plasmid

#205496

Purpose

Expression of hfYFP-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

hfYFP-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW Fold6-T2A-mCherry

Plasmid

#205492

Purpose

Expression of Fold6-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Fold6-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mfRFP-T2A-mtq2

Plasmid

#205494

Purpose

Expression of mfRFP-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mfRFP-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW yoTagRFP657-T2A-mtq2

Plasmid

#205505

Purpose

Expression of yoTagRFP657-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

yoTagRFP657-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFP2-T2A-mtq2

Plasmid

#205503

Purpose

Expression of miRFP2-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP2-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFp670nano3-T2A-mtq2

Plasmid

#205501

Purpose

Expression of miRFp670nano3-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFp670nano3-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFP670-T2A-mtq2

Plasmid

#205491

Purpose

Expression of miRFP670-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP670-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAXY0019

Plasmid

#205992

Purpose

pNpuDnaE(C)_HygR(53-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD424-AP-4 σ4

Plasmid

#198328

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-4 σ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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