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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,237 results for: 272

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Showing: 2261 - 2280 of 4237 results

  2. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  7. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

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  12. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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  20. A3A-PBE

    Plasmid
    #119768
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A and E945K in wild type …
    Promoter
    maize Ubiquitin-1
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119768-plasmid-map-sequence-id-232090

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Showing: 2261 - 2280 of 4237 results