We narrowed to 13,812 results for: cas9 genes

1. p2T-CMV-evoCjCas9-ABE8e-BlastR

   Plasmid

   #194060

   Purpose

   Mammalian expression of evoCjCas9 ABE8e

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

   Plasmid

   #64512

   Purpose

   Expression of St1 dCas9-3XTagBFP2 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

   Plasmid

   #98971

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNA and spCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSHS207 - Bacterial expression plasmid for WT SpCas9

   Plasmid

   #101199

   Purpose

   Bacterial expression plasmid for WT SpCas9

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CASI-Cas9C-P2A-turboGFP

   Plasmid

   #80942

   Purpose

   Expresses Cas9C in mammalian cells; co-expresses turboGFP.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

   Plasmid

   #159917

   Purpose

   Mutagenesis of Drd1

   Depositor

   Larry Zweifel

   Insert

   Drd1 (Drd1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

    Plasmid

    #129026

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EFS-CjCas9-eGFP-HIF1a

    Plasmid

    #137929

    Purpose

    Expression of cjCas9 with Hif1a targeting sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40NLS-HA-T2A-EGFP

    Expression

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sox1/2/3-2373/+3>nls::Cas9::nls

    Plasmid

    #59989

    Purpose

    Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Sox1/2/3 (SoxB1) -2373/+3

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    pU6 5'CMV and EF1-alpha

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-SauriCas9-KKH-Puro

    Plasmid

    #135966

    Purpose

    Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid

    #192668

    Purpose

    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    TRE3G

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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