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pEF5/FRT-DEST EVC2ala4-Flag

Plasmid

#41008

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed amino acids 1183-1186 to Alanine

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
pLV-hPGK-DEST-IRES-Puro

Plasmid

#253773

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-Puro

Plasmid

#253769

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-DEST-IRES-Puro

Plasmid

#253777

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to puromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-DEST-IRES-Neo

Plasmid

#253776

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-DEST-IRES-EGFP

Plasmid

#253778

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human UbC promoter for expression of a gene of interest linked to EGFP by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-Neo

Plasmid

#253772

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-Hygro

Plasmid

#253767

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1alpha-DEST-IRES-Neo

Plasmid

#253768

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-hPGK-DEST-IRES-Hygro

Plasmid

#253771

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a hPGK promoter for expression of a gene of interest linked to hygromycin selection by an IRES.

Depositor

Samuel McBrayer

Article

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DEST N-HPV101 E7

Plasmid

#253677

Purpose

Expresses N-Flag/HA HPV101 E7 in lentiviral background

Depositor

Karl Munger

Article

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Use

Lentiviral

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DEST C-HPV101 E7

Plasmid

#253676

Purpose

Expresses C-Flag/HA HPV101 E7 in lentiviral background

Depositor

Karl Munger

Article

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Use

Lentiviral

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST14-His6-TEV-SpyCatcher003-cys

Plasmid

#249543

Purpose

Expresses SpyCatcher003 with a N-terminal 6x-His tag and a C-terminal cysteine in E.coli under the T7 promoter

Depositor

Kai Zinn

Article

Anaya et al bioRxiv 2025.07.09.663943

Insert

SpyCatcher003-cys

Tags

6x His and Cysteine

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pMC-Dest-uni-gRNA5-BsaI

Plasmid

#241150

Purpose

Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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EKv444(pIhcsrI677-Cas9 GG destination vector )

Plasmid

#248344

Purpose

Golden Gate destination vector containing S. pyogenes Cas9 nuclease (with BsaI sites removed). For multicassette gRNA assembly and expression in moss Physcomitrium patens (Physcomitrella).

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN11(D425A,C459S)-Clover

Plasmid

#215526

Purpose

Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-hSTARR-luc-Pmyc-ccw

Plasmid

#158027

Purpose

This is a dual function destination vector for eSTARR-seq and luciferase assay to quantify enhancer activity. It contains a MYC promoter, a luciferase CDS, followed by a Gateway cassette (R2-R1).

Depositor

Haiyuan Yu

Article

Tippens et al Nat Genet. 2020 Oct;52(10):1067-1075. do

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-dest-BsaI-cmlc2:mCherry

Plasmid

#241213

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12(D199A,C231S)-Clover

Plasmid

#215528

Purpose

Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLPC-C-SF-TAP-DEST-TLK1- M566T

Plasmid

#218831

Purpose

Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

Depositor

Travis Stracker

Insert

TLK1 M566T (TLK1 Human)

Tags

FLAG-STREP

Expression

Mammalian

Mutation

M566T

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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