We narrowed to 32,527 results for: Eng

1. pNLF1-C_RFWD3:1-774

   Plasmid

   #210955

   Purpose

   Mammalian expression of full-length human RFWD3. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   RFWD3:M1-E774 (RFWD3 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLF1-C_WDR55:1-383

   Plasmid

   #210957

   Purpose

   Mammalian expression of full-length human WDR55. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR55:M1-D383 (WDR55 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   C151R natural variant (VAR_037057)

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNLF1-C_WDR12:1-423

   Plasmid

   #210953

   Purpose

   Mammalian expression of full-length human WDR12. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR12:M1-A423 (WDR12 Human)
   

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiT3.1-C_WDR89:1-387

   Plasmid

   #211049

   Purpose

   Mammalian expression of full-length human WDR89. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR89:M1-Q387 (WDR89 Human)
   

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)

   Plasmid

   #71814

   Purpose

   Expresses high specificity SpCas9. Px330-like plasmid.

   Depositor

   Feng Zhang

   Article

   Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

   Has Service

   Cloning Grade DNA

   Insert

   enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-F-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140274

   Purpose

   F-RAM, the Fos-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-11b_PcrA_M6_H93A

   Plasmid

   #194156

   Purpose

   Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

   Depositor

   Taekjip Ha

   Article

   Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

   Insert

   Engineered helicase PcrA_M6
   

   Tags

   6x His tag and FLAG tag

   Expression

   Bacterial

   Mutation

   H93A, C96A, N187C, C247A, L384V, L409C

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK527a

    Plasmid

    #110606

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pV132-vCas9

    Plasmid

    #203223

    Purpose

    Mammalian expression of vCas9

    Depositor

    Robert Langer, Phil Sharp, Vikash Chauhan

    Article

    Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    vCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    S55R R976A K1003A T1314R

    Promoter

    CBh

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-B1

    Plasmid

    #31705

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-106a
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid

    #164514

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Mutation

    Synthetic Construct

    Promoter

    GFAP

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid

    #164510

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Has Service

    AAV9

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Mutation

    Synthetic Construct

    Promoter

    Synapsin

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1-H-Luc_nucl

    Plasmid

    #113931

    Purpose

    Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_nucl
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF.hICN1.CMV.GFP

    Plasmid

    #17623

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYL-kasOp-FnCas12a3

    Plasmid

    #177875

    Purpose

    Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

    Depositor

    Yunzi Luo

    Article

    Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

    Insert

    Streptomyces codon optimized the FnCas12a mutant EP16
    

    Use

    CRISPR

    Promoter

    kasOp*

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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