We narrowed to 32,079 results for: Eng

1. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-11b_PcrA_M6_H93A

   Plasmid

   #194156

   Purpose

   Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

   Depositor

   Taekjip Ha

   Article

   Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

   Insert

   Engineered helicase PcrA_M6
   

   Use

   Tags

   6x His tag and FLAG tag

   Expression

   Bacterial

   Mutation

   H93A, C96A, N187C, C247A, L384V, L409C

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF.hICN1.CMV.GFP

   Plasmid

   #17623

   Purpose

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   ICN1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYL-kasOp-FnCas12a2

   Plasmid

   #177874

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OA984

   Plasmid

   #120363

   Purpose

   Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

   Depositor

   Omar Akbari

   Article

   Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

   Insert

   1C19 single chain antibody
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   AAEL010782 carboxypeptidase A (CPA) gene Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-INPP5B

   Plasmid

   #171947

   Purpose

   Expresses INPP5B in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   INPP5B (INPP5B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3.1-H-Luc_nucl

   Plasmid

   #113931

   Purpose

   Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc_nucl
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tol2-lyzC-mito-Dendra2

    Plasmid

    #107590

    Purpose

    Expresses mitochondria localized Dendra2 in zebrafish neutrophils

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    mito-Dendra2
    

    Use

    Tags

    mitochondira localization sequence

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hsyn-flex-Positron-ST

    Plasmid

    #129267

    Purpose

    Cre-dependant expression of soma-localized Positron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    hsyn

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1-S-Luc_cyto

    Plasmid

    #113926

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGY53

    Plasmid

    #221709

    Purpose

    Empty Cas12a vector for multiplex gene editing in fungi

    Depositor

    Kyle Pomraning

    Article

    Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

    Type

    Empty backbone

    Use

    CRISPR; Aspergillus

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC-H1-gRNA

    Plasmid

    #61089

    Purpose

    CRISPR/Cas9 system gRNA cloning

    Depositor

    Shenglin Huang

    Article

    Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

    Insert

    H1 promoter; gRNA sequences
    

    Use

    CRISPR; /cas9 grna cloning vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSIBR003

    Plasmid

    #177666

    Purpose

    SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ133-RZ-Cas12j2

    Plasmid

    #189784

    Purpose

    Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge