We narrowed to 26,369 results for: vit;

1. px335 Mettl14 sgRNA #2

   Plasmid

   #61514

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gccgctcccggatctcctgc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. STARR-seq luciferase INF enhancer vector_ORI_ISG15

   Plasmid

   #99322

   Purpose

   Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr1: 947976 -949995 (ISG15 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastbac-Flag-BRCA1

   Plasmid

   #223228

   Purpose

   Insect expression construct for Flag-tagged BRCA1 protein production and purification

   Depositor

   Weixing Zhao

   Article

   Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

   Insert

   Breast Cancer 1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available Since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-CFP-KRAS4B

   Plasmid

   #112717

   Purpose

   Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

   Depositor

   Kenneth Westover

   Article

   Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

   Insert

   CFP-KRAS4B (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. px335 Mettl14 sgRNA #1

   Plasmid

   #61515

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gcggcagctcctagctcagc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. STARR-seq luciferase validation vector_ORI_AGAP1

   Plasmid

   #99300

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. STARR-seq luciferase validation vector_mP_AGAP1

   Plasmid

   #99301

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. STARR-seq luciferase validation vector_SCP1_AGAP1

   Plasmid

   #99302

   Purpose

   Luciferase validation vector with AGAP1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   enhancer; chr2: 236735694-236737193 (AGAP1 Human)
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBRPyCAG-Mettl3-dsRed-IRES-puro

   Plasmid

   #61516

   Purpose

   Constitutive expression of murine wild-type Mettl3

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGG

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-TRI-2A

    Plasmid

    #74674

    Purpose

    mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Inserts

    HO1 (HMOX1 Human)
    CD73 (NT5E Human)
    CD39 (ENTPD1 Human)
    

    Use

    Tags

    F2A

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_ spike_del19

    Plasmid

    #155297

    Purpose

    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

    Depositor

    Raffaele De Francesco

    Article

    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

    Insert

    SARS-Cov2 spike_deleted (S SARS-Cov2)
    

    Use

    Generation of sars-cov-2-pseudotyped lentiviral p…

    Tags

    Expression

    Mutation

    Deletion in C-term 19 aa of spike

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMDW139

    Plasmid

    #227336

    Purpose

    Template for in vitro Pol II transcription

    Depositor

    Michelle Wang

    Insert

    Gap DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB514-35S:VrFLS2-HA

    Plasmid

    #226415

    Purpose

    Expressing Vitis riparia FLS2 protein in plants

    Depositor

    Gitta Coaker

    Insert

    VrFLS2
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIVTRup

    Plasmid

    #101362

    Purpose

    Highly efficient in vitro transcription. Empty backbone.

    Depositor

    Ángel Raya

    Insert

    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    

    Use

    For cloning a gene for in vitro transcription pur…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT18mobsacB

    Plasmid

    #72648

    Purpose

    sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGWB514-35S:FLS2XL-HA

    Plasmid

    #226412

    Purpose

    Expressing Vitis riparia FLS2XL (FLS2 with eXtended Ligand recognition) protein in plants

    Depositor

    Gitta Coaker

    Insert

    FLS2XL
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST2T18ms

    Plasmid

    #72647

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDONR2T18ms

    Plasmid

    #72645

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pOpen-K12polLF

    Plasmid

    #165511

    Purpose

    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    DNA Polymerase I, Large (Klenow) Fragment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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