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Plasmid#124785PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorInsertNod-BFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP159
Plasmid#106361PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to GFP-labelled RAB-11 expressed specifically in the epidermis.DepositorInsertttTi5605_SEC_dpy-7p_GFP_RAB-11_3'unc-54
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaAD53A
Plasmid#101831Purposedrives expression of rpaA D53A variant from an rpaA-independent promoterDepositorInsertRpaA D53A with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationD53 mutated to APromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p218 pCMV-CREM-L
Plasmid#8396DepositorInsertCREM-L
UseCre/LoxTagsExpressionMammalianMutationmodified Cre, single LOX 511 sitePromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -