We narrowed to 43,154 results for: cha

1. pNLF1-N_DCAF1:1038-1400

   Plasmid

   #210962

   Purpose

   Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF1:E1038-E1400 (DCAF1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNLF1-N_DCAF15:1-600

   Plasmid

   #210958

   Purpose

   Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   DCAF15:M1-L600 (DCAF15 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNLF1-C_FBXW11:1-542

   Plasmid

   #210952

   Purpose

   Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   FBXW11:M1-R542 (FBXW11 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBiT3.1-N_SKIC8:1-305

   Plasmid

   #211038

   Purpose

   Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   SKIC8:M1-I305 (SKIC8 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBiT3.1-C_COPB2:1-597

   Plasmid

   #211055

   Purpose

   Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   COPB2:M1-A597 (COPB2 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBiT3.1-N_RBBP4:1-425

   Plasmid

   #211037

   Purpose

   Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   RBBP4:M1-S425 (RBBP4 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cav2.2 W391A pcDNA3

   Plasmid

   #206085

   Purpose

   expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

   Plasmid

   #106469

   Purpose

   Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W(S219,D415,H474A)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Changed Serine 219, Aspartate 415 and Histidine 4…

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CYLBL_Neo_CAGdCas9-KRAB

   Plasmid

   #220439

   Purpose

   CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

   Depositor

   Yin Shen

   Article

   Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

   Insert

   dCas9
   

   Use

   CRISPR and TALEN

   Tags

   KRAB

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid

    #91819

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNLF1-C_PLCG1:1-1291

    Plasmid

    #211089

    Purpose

    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    PLCG1:M1-L1291 (PLCG1 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    isoform 2. I813T natural variant (VAR_011908)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBiT3.1-N_FBXW7:1-707

    Plasmid

    #211099

    Purpose

    Mammalian expression of full-length human FBXW7. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBiT3.1-C_FBXW7:1-707

    Plasmid

    #211104

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNLF1-N_CCT5:1-541

    Plasmid

    #210978

    Purpose

    Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    CCT5:M1-E541 (CCT5 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNLF1-N_FBXW7:1-707

    Plasmid

    #210946

    Purpose

    Mammalian expression of full-length human FBXW7. N-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFBOH-LIC_DDB1:1-1140_delta:396-689

    Plasmid

    #210897

    Purpose

    Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    loop deletion amino acids I396 to D689

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pNLF1-C_FBXW7:1-707

    Plasmid

    #210951

    Purpose

    Mammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    FBXW7:M1-K707 (FBXW7 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFBOH-MHL_EIF3B:127-814

    Plasmid

    #210909

    Purpose

    Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    EIF3B:D127-E814 (EIF3B Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQGMQ

    Expression

    Insect

    Mutation

    Y760C sequence conflict

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pFBOH-avi-TEV-LIC_DCAF12:1-453

    Plasmid

    #210940

    Purpose

    Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    Polyhedrin

    Available since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFBOH-MHL_EML4:250-877

    Plasmid

    #210914

    Purpose

    Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    EML4:I250-A877 (EML4 Human)
    

    Use

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Insect

    Mutation

    K283E natural variant (VAR_031726), I382V Natural…

    Promoter

    Polyhedrin

    Available since

    March 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge