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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00727

Plasmid

#39748

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9932 (CG9932 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-AlstR-IRES2-EGFP

Plasmid

#14895

Depositor

Edward Callaway

Article

Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

Insert

AlstR (AstA-R1 Fly)

Use

AAV

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSlmb-vhhGFP4

Plasmid

#35577

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pEJS2437_Nme2.Smu-ABE8e-nt

Plasmid

#201519

Purpose

Mammalian expression of N-terminally fused Nme2.Smu-ABE8e-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2.Smu-ABE8e-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

Plasmid

#63167

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-cMyc"

Tags

N-myristoylation

Expression

Insect

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG

Plasmid

#63169

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-FLAG"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab32

Plasmid

#31744

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab32 (Rab32 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB1-2-PT-SA-SD-1-GFP11

Plasmid

#172068

Purpose

Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUAST td tomato Rab5 WT

Plasmid

#58446

Purpose

expression of drosophila td tomato Rab5

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Tags

td tomato

Expression

Insect

Promoter

GAL4

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS Exon Vector

Plasmid

#69884

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pKC52

Plasmid

#226780

Purpose

Expresses Drosophila UNC-104(R348G) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R348G) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 348 to Glycine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKC50

Plasmid

#226778

Purpose

Expresses Drosophila UNC-104(V6M) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (V6M) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Valine 6 to Methionine

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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