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pCMV-GCaMP6fu

Plasmid

#86235

Purpose

Express fluorescent calcium indicator GCaMP6fu in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

Insert

GCaMP6fu

Expression

Mammalian

Mutation

W43Y D395A

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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PEX-LV

Plasmid

#12120

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

noncatalytic fragment of MMP2 comprised of the fi…

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
Ateam1.03-nD/nA/pRSETB

Plasmid

#51960

Purpose

bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Tags

6xHis

Expression

Bacterial

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CMV:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135461

Purpose

Mammalian expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

CMV

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_YR_1.0

Plasmid

#59801

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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Ateam1.03-nD/nA/pENTR1A

Plasmid

#51959

Purpose

Gateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Entry vector

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Orange_CaMBI_300

Plasmid

#124095

Purpose

orange calcium-modulated bioluminescent indicator, 300nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 300

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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rArc

Plasmid

#178206

Purpose

Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

Depositor

Stefan Heinemann

Article

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rArc

Expression

Mammalian

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHK352.3

Plasmid

#235473

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone RSF1030)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Tags

Golgi and ER export signal

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pJBM3

Plasmid

#225290

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC388 for pJBM3

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Blue_CaMBI

Plasmid

#124097

Purpose

Blue calcium-modulated bioluminescent indicator

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Blue CaMBI

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Green_CaMBI

Plasmid

#124096

Purpose

Green calcium-modulated bioluminescent indicator

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Green CaMBI

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_CY_1.0

Plasmid

#59800

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-HASAP1-ST

Plasmid

#138965

Purpose

chemigenetic voltage indicator, soma targeted

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP1-ST

Tags

somatic localization tag

Expression

Mammalian

Promoter

CAG

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET42a-lambdaN+-L+-GSH

Plasmid

#98894

Purpose

Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

lambdaN+-L+

Tags

GST tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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