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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-DreERT2 (JDW 196)

Plasmid

#242586

Purpose

The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-human Cyclin L1 alpha-IRES-EGFP

Plasmid

#240140

Purpose

Express Cyclin L1 alpha

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 alpha (CCNL1 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1508)

Plasmid

#242555

Purpose

Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-HYGRO-P2A-mAID-mCLOVER-LRR1

Plasmid

#221546

Purpose

DONOR homology arm-containing repair template used to insert Auxin-Inducible Degron tags, mClover, and Hygromycin resistance markers to the N-terminus of LRR1

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

LRR1 (LRR1 Human)

Use

Unspecified

Tags

HYGRO-P2A-mAID-mCLOVER

Promoter

N/A

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hIRF5-V5(4D)-LPETG

Plasmid

#237225

Purpose

Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Interferon regulatory factor 5 (IRF5 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S451D, S453D, S456D, S462D

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-hSema4D-Q497P

Plasmid

#190655

Purpose

Expresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

myc

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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