We narrowed to 2,968 results for: GRI;

1. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174647

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   CMV (with TetOn)

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57.1 DOX off blast NFS1

   Plasmid

   #160801

   Purpose

   Express Dox repressible NFS1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   NFS1 (NFS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tet-pLKO-puro shPOLE1_1

   Plasmid

   #160810

   Purpose

   Express Dox repressible shPOLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE1_1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

   Plasmid

   #166809

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUMO His6 SUMO SnRK2.2 (20-362)

   Plasmid

   #177845

   Purpose

   Bacterial Expression of SnRK2.2

   Depositor

   Karsten Melcher

   Article

   Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIS54 ADAR 3'UTR mut

   Plasmid

   #14488

   Depositor

   David Bartel

   Article

   Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

   Insert

   ADAR 3'UTR mutant (ADAR Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   CATTCC’s were changed to AAGTAC's

   Promoter

   Available since

   March 7, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

   Plasmid

   #166801

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   S730A

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG71 BDNF 3'UTR mut

   Plasmid

   #12054

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   BDNF 3'UTR mut (BDNF Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Promoter

   Available since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.TRC1.shmNsd1.2, puro

   Plasmid

   #114447

   Purpose

   Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

   Depositor

   Adrian Bracken

   Article

   Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

   Insert

   shNsd1.2 (Nsd1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.TRC1.shmNsd1.1, puro

    Plasmid

    #114446

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.1 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid

    #174628

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(nano)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

    Plasmid

    #113041

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid

    #174629

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG30 IQGAP1 3'UTR mut

    Plasmid

    #14504

    Depositor

    David Bartel

    Article

    Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

    Insert

    IQGAP1 3'UTR mutant (IQGAP1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    GTGCCTT was changed to GTGCGCC and GTGCCT was cha…

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

16. mCherry-FKBP-TPD54(L53P,L67P)

    Plasmid

    #172458

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Leucines 53 and 67 to proline (breaks coi…

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

    Plasmid

    #113040

    Purpose

    AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

    Plasmid

    #113042

    Purpose

    AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S KK93EE

    Plasmid

    #166796

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. KK93EE mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    KK93EE

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S E597R

    Plasmid

    #166800

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. E597R mutant is partial loss of CARD8 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    E597R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge