We narrowed to 7,283 results for: gla

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pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-27

Plasmid

#172752

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-27

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXylA-RgTALsyn

Plasmid

#100948

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

XylA (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pXylA-RgTALsyn

Plasmid

#100948

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

XylA (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-RH-mycStrep

Plasmid

#31839

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R155H

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-8

Plasmid

#172761

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-8

Plasmid

#172761

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-5

Plasmid

#172760

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-5

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG-5

Plasmid

#172760

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-5

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD71Cas10.1-Pct5.1-crRNA(dadR)

Plasmid

#191634

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD71Cas10.1-Pct5.1-crRNA(dadR)

Plasmid

#191634

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge