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pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

Plasmid

#164266

Purpose

sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6 promoter from W05B2.8

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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L4278 hIgKLSS_hTGFb1 in pcDNA backbone

Plasmid

#244184

Purpose

Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TGF-beta1 with IgK light chain secretion sequence (TGFB1 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLUEF1 IRES GFP (backbone)

Plasmid

#224466

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Type

Empty backbone

Use

Lentiviral

Promoter

Ef1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - vector backbone

Plasmid

#233132

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Type

Empty backbone

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-S1S2 [rev-backbone]

Plasmid

#229199

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-NbON10-NanoLuc

Plasmid

#198691

Purpose

Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, IgK leader sequence, NanoLuc, and twin…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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