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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,107 results for: gibson assembly

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Showing: 221 - 240 of 1107 results

  14. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

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  15. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  16. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Mutation
    BsaI site removed
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120972-plasmid-map-sequence-id-236024

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  17. pHS2002

    Plasmid
    #239832
    Purpose
    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    NovaIscB omegaRNA scaffold
    Expression
    Mammalian
    Mutation
    Replacement of A12-A57 with GAAA in omegaRNA scaf…
    Promoter
    human U6
    Available Since
    July 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239832-plasmid-map-sequence-id-479110

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  18. pNS3

    Plasmid
    #131770
    Purpose
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites
    Depositor
    Jared Toettcher
    Article
    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d
    Insert
    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    Use
    Integration into δ-sites
    Expression
    Yeast
    Mutation
    E490G to make Cry2 into Cry2Olig
    Promoter
    PADH1
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    131770-plasmid-map-sequence-id-257191

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Showing: 221 - 240 of 1107 results