We narrowed to 6,822 results for: pcdna3.1

Showing: 2381 - 2400 of 6822 results

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pCDNA3.1-HsPcdh21-Myc-Flag-His

Plasmid

#210818

Purpose

Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3x Flag, 10x His

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

Plasmid

#193997

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181_NLS

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181_NLS

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

Plasmid

#171785

Purpose

Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-hH2A

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-hH2A-(GGS)X6-HaloTag C58

Plasmid

#171431

Purpose

Expression of hH2A-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

hH2A-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C261

Plasmid

#171429

Purpose

Expression of FKBP-(GGS)X6-HaloTagM C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C261

Expression

Mammalian

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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(365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)

Plasmid

#168188

Purpose

HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

Plasmid

#127314

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

Plasmid

#228401

Purpose

Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

SREBP1 (SREBF1 Human)

Tags

mNeonGreen2x

Expression

Mammalian

Mutation

deleted N terminal activation domain (amino acids…

Promoter

EF1a

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187089

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Plasmid

#160742

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

Plasmid

#160741

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

Plasmid

#163990

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

Plasmid

#111459

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

Ha Tag

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

Plasmid

#69903

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

Plasmid

#49064

Purpose

Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Article

Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

Insert

human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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XE250 pCDNA3.1 + (zeo)- VSV-KLHL12-Q405X

Plasmid

#16759

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

VSV-KLHL12-Q405X (KLHL12 Human)

Tags

vsv

Expression

Mammalian

Mutation

Q405STOP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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