We narrowed to 3,490 results for: ttl

1. pJB43

   Plasmid

   #226302

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   WT CsoS2, frameshift site intact
   

   Expression

   Bacterial

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBM1

   Plasmid

   #225288

   Purpose

   E. coli- C. coli shuttle vector; KanR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Replication elements from pWestmill 11
   

   Expression

   Bacterial

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 msNLRP3 (STOP)

   Plasmid

   #218638

   Purpose

   Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ301

   Plasmid

   #205415

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

   Insert

   Hs1Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Expression

   Plant

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSM-dCas9::Ap

   Plasmid

   #154307

   Purpose

   Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   I-Son Ng

   Article

   Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-Cka2

   Plasmid

   #99980

   Purpose

   Shuttle yeast Cka2 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Cka2 (CKA2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-Utp16

   Plasmid

   #99985

   Purpose

   Shuttle yeast Utp16 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Utp16 (BUD21 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJYS2_crtYf

   Plasmid

   #85544

   Purpose

   Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Expression

   Bacterial

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-M2

    Plasmid

    #135583

    Purpose

    High eukaryotic protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYHY2

    Plasmid

    #216108

    Purpose

    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT0294 signal peptide

    Expression

    Bacterial

    Promoter

    BfP1E6 promoter

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX-TRV1

    Plasmid

    #180515

    Purpose

    Agrobacterium tumefaciens-based expression of TRV1

    Depositor

    Jose-Antonio Daros

    Article

    Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

    Insert

    Tobacco rattle virus RNA1
    

    Expression

    Plant

    Promoter

    35S CaMV

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRBBm34

    Plasmid

    #48114

    Purpose

    Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    gfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Expression

    Bacterial

    Promoter

    pLacI

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pESC-leu2d(empty)

    Plasmid

    #20120

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. p201G Cas9

    Plasmid

    #59178

    Purpose

    Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    sGFP
    

    Use

    CRISPR

    Promoter

    2x35S

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSV2_PflaB-mCherryBb

    Plasmid

    #118243

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven mCherry
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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