We narrowed to 5,282 results for: golden gate
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Plasmid#231387PurposeFor crRNA cloning with LbCas12a vectors for genome editing in ArabidopsisDepositorInsertU6-29
UseCRISPRExpressionPlantAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227890PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertTOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pR-mAmetrine
Plasmid#228598PurposeMammalian expression of mAmetrine, for MuSIC barcode constructionDepositorInsertmAmetrine
ExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW91013
Plasmid#222450PurposeLevel 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.DepositorInsertCBS4-miniDFR::RUBY::OCS
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFv-HaloTag-iLID
Plasmid#227390PurposeAdhesion module, scFv binds SunTagDepositorInsertscFv-HaloTag-iLID
UseLentiviralAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-hyg9si
Plasmid#222832PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ELP[V]60
Plasmid#219406Purpose(VPGVG)60DepositorInsertELP[V]60
Tags6xHisExpressionBacterialAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP01-UTC-7.0
Plasmid#216625PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test constructDepositorInsertT7p-5'g10-NanoLuc-3'TMV
UseSynthetic BiologyPromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD189 VP16-ZF37
Plasmid#138759PurposeConstitutive expression of VP16-ZF37 under the CMV promoter.DepositorInsertVP16-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH183
Plasmid#122856PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH204
Plasmid#122860PurposeBinary expression vector containing 35S:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modulesDepositorInsert35S:MS2-VP64:tRBCS
UseGolden gate compatible plant transformation vectorAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ralstonia-GG-Kit
Plasmid kit#1000000142PurposePlasmids for gene knockout and complementation in the bacterial plant pathogen Ralstonia solanacearum.DepositorApplicationCloning and Synthetic BiologyVector typeBacterial ExpressionCloning typeGolden GateAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGM000
Plasmid#48864PurposeEmpty entry vector for creating GreenGate supermodules w/o plant resistance cassette.DepositorTypeEmpty backboneUseGolden gate compatible cloning vectorAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGN000
Plasmid#48865PurposeEmpty entry vector for creating GreenGate supermodules with plant resistance cassette.DepositorTypeEmpty backboneUseGolden gate compatible cloning vectorAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by