We narrowed to 7,247 results for: golden gate

1. pR6K-GFP-RFP

   Plasmid

   #199652

   Purpose

   R6K backbone with Golden Gate (GG) cloning sites

   Depositor

   IIya Finkelstein

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pR6K-GFP-RFP

   Plasmid

   #199652

   Purpose

   R6K backbone with Golden Gate (GG) cloning sites

   Depositor

   IIya Finkelstein

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U25m

   Plasmid

   #121027

   Purpose

   MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   RiboJ + UTR1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U25m

   Plasmid

   #121027

   Purpose

   MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   RiboJ + UTR1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. T16m

   Plasmid

   #121035

   Purpose

   MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   L3S2P55 (synthetic Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T16m

   Plasmid

   #121035

   Purpose

   MoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   L3S2P55 (synthetic Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. T17m

   Plasmid

   #121036

   Purpose

   MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   L3S2P11 (synthetic Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T17m

   Plasmid

   #121036

   Purpose

   MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   L3S2P11 (synthetic Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T12m

   Plasmid

   #121031

   Purpose

   MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ECK120029600 (strongest natural Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. T12m

    Plasmid

    #121031

    Purpose

    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ECK120029600 (strongest natural Voigt terminator)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

    Plasmid

    #242152

    Purpose

    Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170

    Depositor

    Dominik Niopek

    Insert

    AraC R2 S170 LOV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E3K, R146L, A152T, R251L

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDest_PANCEd_AraC_S170_LOV_sd2_R2_C1

    Plasmid

    #242152

    Purpose

    Expression Plasmid containing a LacI repressed sd2 AraC R2 PANCE cassette with a LOV domain insertion at S170

    Depositor

    Dominik Niopek

    Insert

    AraC R2 S170 LOV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E3K, R146L, A152T, R251L

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

    Plasmid

    #242153

    Purpose

    Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170

    Depositor

    Dominik Niopek

    Insert

    AraC R5 S170 LOV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A2E, L156I, R251H

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDest_PANCEd_AraC_S170_LOV_sd2_R5_C1

    Plasmid

    #242153

    Purpose

    Expression Plasmid containing a LacI repressed sd2 AraC R5 PANCE cassette with a LOV domain insertion at S170

    Depositor

    Dominik Niopek

    Insert

    AraC R5 S170 LOV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A2E, L156I, R251H

    Promoter

    Available since

    Aug. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKS1603

    Plasmid

    #239527

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS1603

    Plasmid

    #239527

    Purpose

    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS1616

    Plasmid

    #239547

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS1616

    Plasmid

    #239547

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS1615

    Plasmid

    #239546

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKS1615

    Plasmid

    #239546

    Purpose

    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    CrG8H-2xCrk_SH3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge