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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2441 - 2460 of 5523 results

  6. pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252560
    Purpose
    Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector
    Depositor
    Kate Galloway
    Publication
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252560-plasmid-map-sequence-id-503972

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  8. pTL5_cHS4-CAG-s36GFPmNG-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4

    Plasmid
    #223540
    Purpose
    PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells
    Depositor
    Sally Lowell
    Publication
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, mCherry-3NLS, PAC
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223540-plasmid-map-sequence-id-508850

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  14. pSLQ14902

    Plasmid
    #239277
    Purpose
    Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO
    Depositor
    Stanley Qi
    Publication
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PspCas13b-hGAPDH-gRNA2 (GAPDH Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239277-plasmid-map-sequence-id-474373

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  15. yeast Tunable Expression Systems Toolkit (yTEST)

    Plasmid kit
    #1000000266
    Purpose
    Plasmids for assembly of small-molecule inducible promoter systems in Saccharomyces cerevisiae.
    Depositor
    Jeff Hasty
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only

  17. Tad-CBE(TCA3)

    Plasmid
    #235064
    Purpose
    Expresses Tad-CBE(TCA3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCA3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235064-plasmid-map-sequence-id-466802

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Showing: 2441 - 2460 of 5523 results