We narrowed to 23,481 results for: IRE

1. pJAF213

   Plasmid

   #52403

   Purpose

   Expresses Flag-tagged mouse Arf2

   Depositor

   Gregory Pazour

   Article

   Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

   Insert

   Arf2 (Arf2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAc5.1B-lambdaN-HA-DmGW182

   Plasmid

   #22406

   Depositor

   Elisa Izaurralde

   Article

   Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

   Insert

   gawky, variant A (gw Fly)
   

   Use

   Drosophila expression

   Tags

   lambdaN-HA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSuper mouse DGCR8-3

   Plasmid

   #14774

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 Apr 1. ():.

   Insert

   DGCR8 shRNA (Dgcr8 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

4. CS2-GFP-Ben

   Plasmid

   #47966

   Purpose

   Expression of fly Ben

   Depositor

   Laura Lee

   Article

   Merkle et al Development. 2009 Feb;136(3):449-59. doi

   Insert

   Bendless (ben Fly)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. HA PRAS40 F129A

   Plasmid

   #15673

   Depositor

   John Lawrence

   Article

   Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

   Insert

   PRAS40 F129A (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F129A

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pSicoR (GFP) cRel shRNA1

   Plasmid

   #22509

   Depositor

   Tyler Jacks

   Article

   Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

   Insert

   cRel shRNA1 (Rel Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RhoU

   Plasmid

   #55698

   Purpose

   expression of mouse RhoU

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoU (Rhou Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. Pr-deCFP-MGapt

   Plasmid

   #67740

   Purpose

   OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-deCFP-MGapt-T500
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW-EGFP-4xlet-7 (pAW-AS27)

   Plasmid

   #40834

   Purpose

   miRNA sensor used in Drosophila cell lines

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   Four target sites perfectly complementary to let-7
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Pr1-T7RNAP

    Plasmid

    #67738

    Purpose

    OR2-OR1-Pr1 promoter expressing T7 RNAP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-T7RNAP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr1

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pfli1

    Plasmid

    #22413

    Depositor

    Nathan Lawson

    Article

    Lawson et al Development. 2001 Oct . 128(19):3675-83.

    Insert

    fli1 (fli1 Zebrafish)
    

    Use

    Riboprobe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pNB655

    Plasmid

    #60142

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RhoD

    Plasmid

    #55700

    Purpose

    expression of mouse RhoD

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoD (Rhod Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBS cErm (CT#1054)

    Plasmid

    #13907

    Depositor

    Cliff Tabin

    Article

    Brent et al Development. 2004 Aug . 131(16):3885-96.

    Insert

    Erm (ETV5 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pAc5.1B-EGFP-Dm4E-T

    Plasmid

    #79261

    Purpose

    Expresses EGFP-tagged Dm4E-T in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    Dm4E-T (4E-T Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Pr2-T7RNAP

    Plasmid

    #67739

    Purpose

    OR2-OR1-P2 promoter expressing T7 RNAP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-T7RNAP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr2

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RAP2C

    Plasmid

    #55668

    Purpose

    expression of mouse RAP2C

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RAP2C (Rap2c Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. TU#1450_unc-53a_cDNA

    Plasmid

    #82361

    Purpose

    express unc-53a cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-53 a isoform (unc-53 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSicoR (Puro) cRel shRNA2

    Plasmid

    #22510

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    cRel shRNA2 (Rel Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pBluescript-mHMGB4

    Plasmid

    #31620

    Depositor

    Martin Lotz, Yasuhiko Kawakami

    Article

    Itou et al Dev Dyn. 2011 May . 240(5):1151-62.

    Insert

    HMGB4 (Hmgb4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

21. RhoJ

    Plasmid

    #55696

    Purpose

    expression of mouse RhoJ

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoJ (Rhoj Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

22. myc GbetaL mutant 5

    Plasmid

    #8479

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    GbetaL (MLST8 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G192D

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

23. 6his R.3-AR GUSpET28a+/pLysS

    Plasmid

    #61157

    Purpose

    N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

    Depositor

    Ichiro Matsumura, Andrew Ellington

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    GUSAR
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

    Promoter

    T7

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Mad WT

    Plasmid

    #22996

    Depositor

    Edward De Robertis

    Article

    Eivers et al PLoS One. 2009 . 4(8):e6543.

    Insert

    Mad (Mad Fly)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pBEST-p15A-J23101-deGFP-T500 (131p)

    Plasmid

    #70207

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-Y-GECO1

    Plasmid

    #55767

    Purpose

    Express Y-GECO1 in neurons

    Depositor

    Robert Campbell

    Article

    Zhao et al Integr Biol (Camb). 2014 May 19.

    Insert

    Y-GECO1.0
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mGDF8 donor mCherry-PA

    Plasmid

    #72596

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    mCherry-puromycin cassetter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pGEX-2T FRB R2109A

    Plasmid

    #26609

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    FRB domain only (aa 2015-2114); R2109A

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

29. myc GbetaL mutant 7

    Plasmid

    #8481

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    GbetaL (MLST8 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    F320S

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

30. myc GbetaL mutant 2

    Plasmid

    #8478

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    GbetaL (MLST8 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S72D

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

31. Pr-delRBS-deGFP

    Plasmid

    #67744

    Purpose

    Pr promoter expressing a deGFP that is missing a ribosomal binding site

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    deGFP-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. RASL10A

    Plasmid

    #55674

    Purpose

    expression of mouse RASL10A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RASL10A (Rasl10a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pBS cFgfr2 (CT#221)

    Plasmid

    #14003

    Depositor

    Cliff Tabin

    Article

    Brent et al Development. 2004 Aug . 131(16):3885-96.

    Insert

    Fgfr2 (FGFR2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pATI (pKD-G7)

    Plasmid

    #62686

    Purpose

    Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP123/amiR-eGFP419
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HA PRAS40 P185A

    Plasmid

    #15675

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 P185A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P185A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

36. p3xFLAG-Tmprss6(S762A)

    Plasmid

    #18791

    Depositor

    Bruce Beutler

    Article

    Du et al Science. 2008 May 23. 320(5879):1088-92.

    Insert

    Type II transmembrane serine protease 6 (Tmprss6 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Changed Serine762 to Alanine

    Promoter

    Available since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

37. ARF17 pGreenII0229

    Plasmid

    #12070

    Depositor

    David Bartel

    Article

    Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

    Insert

    ARF17 (ARF17 Mustard Weed)
    

    Use

    Plant transformation via agrobacterium

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. myc GbetaL mutant 1

    Plasmid

    #8477

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    GbetaL (MLST8 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D42A

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. myc GbetaL mutant 6

    Plasmid

    #8480

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    GbetaL (MLST8 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T208D

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pATM (pKD-G11)

    Plasmid

    #62690

    Purpose

    Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    amiR-eGFP123/amiR-eGFP419/tdTomato
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Mix.2-lux M-p53BE

    Plasmid

    #20894

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

    Insert

    -290 Mix.2 M-p53BE promoter luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    point mutation of the p53 binding element (p53BE)…

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

42. XE151 XDpr-CS2+

    Plasmid

    #16771

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    XDpr (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

43. pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p)

    Plasmid

    #70197

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TU#1448_unc-73b_cDNA

    Plasmid

    #82362

    Purpose

    express unc-73b cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 b isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Mad GSK3

    Plasmid

    #22995

    Depositor

    Edward De Robertis

    Article

    Eivers et al PLoS One. 2009 . 4(8):e6543.

    Insert

    Mad (Mad Fly)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S208A, S204A

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

46. Mad MM

    Plasmid

    #22997

    Depositor

    Edward De Robertis

    Article

    Eivers et al PLoS One. 2009 . 4(8):e6543.

    Insert

    Mad (Mad Fly)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S212A

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

47. pAC-EsaR-I70V/D91G

    Plasmid

    #47644

    Purpose

    pAC-EsaR with a208g and a272g mutations in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-I70V/D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Isoleucine 70 to Valine and Aspartic acid…

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

48. RASL10B

    Plasmid

    #55673

    Purpose

    expression of mouse RASL10B

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RASL10B (Rasl10b Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

49. NKIRAS2

    Plasmid

    #55676

    Purpose

    expression of mouse NKIRAS2

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    NKIRAS2 (Nkiras2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

50. REM1

    Plasmid

    #55681

    Purpose

    expression of mouse REM1

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    REM1 (Rem1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only