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pME_B_0_2_020_Pdummy

Plasmid

#235809

Purpose

Promoter dummy sequence used as a baseline for promoter characterization

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Promoter dummy

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_002_OC5'2

Plasmid

#235802

Purpose

5' Operon Connector 2 in the M1 position used to construct operons

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 2

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_003_OC5'3

Plasmid

#235803

Purpose

5' Operon Connector 3 in the M1 position used to construct operons

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 3

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_005_OC5'5

Plasmid

#235805

Purpose

5' Operon Connector 5 in the M1 position used to construct operons

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 5

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_006_OC5'6

Plasmid

#235806

Purpose

5' Operon Connector 6 in the M1 position used to construct operons

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 6

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1-2_001_OC5'1

Plasmid

#235801

Purpose

5' Operon Connector 1 in the M1 position used to construct operons

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Operon Connector 1

Use

Synthetic Biology

Mutation

none

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

Plasmid

#235970

Purpose

Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly

Depositor

Tobias Erb

Publication

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-TREER

Plasmid

#208321

Purpose

Bacterial expression of the His-tagged peptide TREER.

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Publication

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEsg1-ps-6K-Luc

Plasmid

#15395

Depositor

Shinya Yamanaka

Publication

Amano et al BMC Dev Biol. 2006 Feb 28;6:11. doi: 10.

Insert

Upstream region of mouse Esg1 pseudogene

Use

Luciferase

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Publication

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Tags

mCherry

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

Plasmid

#26797

Purpose

Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately.

Depositor

Edward Hsiao

Publication

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Inserts

Rs1 (HTR4 Human)
tTA

Tags

mCherry-P2A

Expression

Mammalian

Mutation

D100A

Promoter

EF1α and short TetO, miniCMV

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Publication

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_SCP1_empty

Plasmid

#99299

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Publication

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pReporter_5

Plasmid

#60566

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_5

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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3xFlag-tdMCP-Erk2 (pErk reporter)

Plasmid

#226167

Purpose

Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-Erk2 (MAPK1 Human)

Tags

3xFlag-tdMCP

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Publication

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PIP5K1C (PIP5K1C Human)

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Publication

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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