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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,523 results for: golden gate

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Showing: 2461 - 2480 of 5523 results

  4. ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

    Plasmid
    #228663
    Purpose
    Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika virus portion of NS2B protease coexpressed with NS3 protease
    Tags
    His-GST-TEV
    Expression
    Bacterial
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    228663-plasmid-map-sequence-id-461604

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  5. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227890
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Publication
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227890-plasmid-map-sequence-id-455730

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  12. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

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  18. pOPS0378

    Plasmid
    #133229
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Publication
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133229-plasmid-map-sequence-id-276542

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  19. pMH183

    Plasmid
    #122856
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122856-plasmid-map-sequence-id-247655

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  20. pMH204

    Plasmid
    #122860
    Purpose
    Binary expression vector containing 35S:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules
    Depositor
    Markus Schmid
    Publication
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    35S:MS2-VP64:tRBCS
    Use
    Golden gate compatible plant transformation vector
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122860-plasmid-map-sequence-id-239401

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Showing: 2461 - 2480 of 5523 results