We narrowed to 5,282 results for: golden gate
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Plasmid#227910PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227904PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCHW91015
Plasmid#222455PurposeLevel 1, Position 2 containing Mas::CUP2-VP16::NOS.DepositorAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW90004
Plasmid#222436PurposeLevel 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.DepositorInsertCBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
UseSynthetic BiologyExpressionPlantMutationnoAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-SunTag(10X)-2A-BFP
Plasmid#227389PurposeAdhesion module, dCas9-SunTagDepositorInsertdCas9-SunTag
UseLentiviralMutationEnzymatically dead Cas9Available sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-GFP-iLID-Ferritin
Plasmid#227376PurposeCondensate module, light-inducible oligomerization 'core'DepositorInsertGFP 'core'
UseLentiviralAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DVA_p15A_AF
Plasmid#217604PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MoBiFC
Plasmid kit#1000000222PurposeModular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector typePlant ExpressionCloning typeGolden Gate (MoClo)Available sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1047
Plasmid#222726PurposepNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NANOS3_cterm
Plasmid#222904PurposeCas9/sgRNA plasmid for targeting NANOS3DepositorPublicationInsertCas9, NANOS3 sgRNA (NANOS3 Synthetic, Human)
UseCRISPRAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv4
Plasmid#221432PurposeLentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNK2657
Plasmid#219741PurposeMoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiensDepositorInserthispidin- synthase from Mycena citricolor
UseSynthetic BiologyAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK5712
Plasmid#219752PurposepGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnH3H_v2 - tAOX
ExpressionYeastAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-SRSF1 siRNA-resitant
Plasmid#218974PurposeVector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)DepositorInsertSRSF1 cDNA
ExpressionMammalianMutationsiRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…PromoterCMVAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9050
Plasmid#219429PurposePapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)DepositorInsertAATG_PapE-4xLF2_AGGT
UseMoclo compatible level 0 moduleAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-pT231 scFv-GFP
Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
ExpressionYeastPromoterGAL1-10Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid kit#1000000238PurposeUniversal Loop (uLoop) assembly system for efficient and cross-kingdom DNA assembly and to build conjugatable episomes using diatom promoters and terminators.DepositorApplicationCloning and Synthetic BiologyVector typeAlgal Expression, Bacterial Expression, Plant Expression, Yeast ExpressionCloning typeGolden Gate (Loop)Available sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only