We narrowed to 4,698 results for: golden gate

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pGGA002

Plasmid

#48813

Purpose

Provides the AP3 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

AP3 promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSBLego

Plasmid

#219967

Purpose

The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

Depositor

Markus Seeger

Article

Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

Type

Empty backbone

Tags

SLEHHHHHH and pelB signal sequence

Expression

Bacterial

Promoter

pBAD

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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L0-I9 PhiC31 GG1-GG4

Plasmid

#195892

Purpose

Level 0 PhiC31 integrase with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31 integrase

Use

Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pYY62-P1int-TP901-GFPdropout

Plasmid

#235773

Purpose

Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Type

Empty backbone

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-PuroR

Plasmid

#232753

Purpose

IVT template for making modRNA encoding the Puromycin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

PuroR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Article

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRDB_345

Plasmid

#231113

Purpose

ABE activity-based selection cloning vector

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_347

Plasmid

#231114

Purpose

CBE activity-based selection cloning vector

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPlantPOP_STARBURST

Plasmid

#241284

Purpose

UBQ10 driven expression of STARBURST, a poly-cistronic fungal bioluminescense pathway, in plants

Depositor

Sean Cutler

Article

Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

STARBURST

Expression

Plant

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ142

Plasmid

#69294

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET29-neoLux1.2

Plasmid

#229952

Purpose

Bacterial expression for de novo luciferase, neoLux1.2

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

neoLux1.2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ146

Plasmid

#69298

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 6 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSWITCH

Plasmid

#222402

Purpose

Expression in Streptococcus pyogenes

Depositor

Anne Botteaux

Article

Schiavolin et al Microbiol Spectr. 2024 Oct 3;12(10):e011

Type

Empty backbone

Expression

Bacterial

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGGA016

Plasmid

#228231

Purpose

Level 0 for 5xOp (GreenGate A module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

5xOp

Use

Synthetic Biology

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mGreenLantern

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR-TEV (integrating)

Plasmid

#247218

Purpose

Plasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11015

Plasmid

#117499

Purpose

FAST-Red selectable marker Golden Gate Level 1 Position 1

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI

Tags

RFP

Expression

Plant

Promoter

AtOLE1

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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