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pminiTol2-4kb wnt3 promoter-Wnt3EGFP

Plasmid

#203793

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Teh et al Development. 2015 Nov 1;142(21):3721-33.

Insert

Wnt3 (wnt3 Zebrafish)

Use

Zebrafish expression

Tags

mEGFP

Promoter

4kb Wnt3 promoter

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A

Plasmid

#226576

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

R119A, F191A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-mCherry

Plasmid

#220351

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-ΔBH4

Plasmid

#220300

Purpose

mammalian expression of Bcl-2-ΔBH4 tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

BH4 deleted

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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CMV5.1-SARS-CoV-2 Nsp3

Plasmid

#204205

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Tags

FLAGx3

Expression

Mammalian

Mutation

none, from obtained clone

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJJR83

Plasmid

#75028

Purpose

mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

mCherry^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized mCherry

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307-VHL(Y98H)-3F-BirA

Plasmid

#220148

Purpose

Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

changed tyrosine 98 to histidine; codon optimized

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS

Plasmid

#216761

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

Plasmid

#203847

Purpose

Flp-dependent expression of Chronos fused to EGFP

Depositor

Ivo Spiegel

Article

Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

Has Service

AAV2

Insert

Chronos

Use

AAV

Tags

EGFP

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-muGFP

Plasmid

#166727

Purpose

AAV, encodes monomeric ultrastable GFP (muGFP)

Depositor

Daniel Scott

Article

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

AAV

Promoter

Chicken Beta Actin

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMM627

Plasmid

#112532

Purpose

pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-mJP-4

Plasmid

#79599

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Tags

2x mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pPBC-G6f

Plasmid

#62811

Purpose

pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6f-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR3b-(V7)

Plasmid

#124881

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)

Tags

HA Tag, His Tag, and NA

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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