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Plasmid#71573PurposeProduces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges aspartate-317 to glycinePromoterrecAAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK382
Plasmid#71574PurposeProduces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)DepositorInsertcitrate synthase
UseTagsExpressionBacterialMutationchanges aspartate-317 to asparagine; silent mutat…PromoterrecAAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK360
Plasmid#71571PurposeProduces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)DepositorInsertacetyl-CoA:oxalate CoA-transferase
UseTagsExpressionBacterialMutationchanges threonine-2 to alaninePromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK385
Plasmid#71572PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK347
Plasmid#71833PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK348
Plasmid#71832PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK193
Plasmid#71834PurposeCloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) regionDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UsePhage lambda cloning vector with genomic dna inse…TagsExpressionMutationadditional N-terminal methionine encoded by this …PromoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1112
Plasmid#63784PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection markerDepositorInserteSapphire-Strep-tag II followed by URA3
UseE. coli cloning vectorTagsSapphire-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-0X750-luxAB-DAS+4
Plasmid#49517PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 siteDepositorInsertP750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only